Detailed information of TA system TA119904

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-Phd
• Location: 390959..391506
• Replicon: chromosome
• Accession: NZ_CP036500
• Organism: Vibrio cholerae strain 20000

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EZZ25_RS16050
• Protein ID: WP_000229316.1
• Coordinates: 391204..391506 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q9KM93
• Locus tag: EZZ25_RS16045
• Protein ID: WP_000861987.1
• Coordinates: 390959..391216 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ25_RS15985	386482..386592	+	111	WP_076025930.1	DUF645 family protein	-
EZZ25_RS15990	386922..387146	+	225	WP_161571222.1	DUF3709 domain-containing protein	-
EZZ25_RS19435	387347..387508	+	162	Protein_387	acetyltransferase	-
EZZ25_RS19440	387594..387765	+	172	Protein_388	DUF645 family protein	-
EZZ25_RS16000	387916..388584	+	669	WP_002033340.1	hypothetical protein	-
EZZ25_RS16005	388581..388697	+	117	WP_088124786.1	DUF3265 domain-containing protein	-
EZZ25_RS19445	388803..388838	+	36	WP_172884631.1	hypothetical protein	-
EZZ25_RS16015	388856..389116	+	261	WP_032468027.1	DUF3709 domain-containing protein	-
EZZ25_RS16025	389364..389735	+	372	WP_001164078.1	nucleotide pyrophosphohydrolase	-
EZZ25_RS16030	389802..390227	+	426	WP_001882332.1	DUF1778 domain-containing protein	-
EZZ25_RS16035	390224..390757	+	534	WP_000118351.1	GNAT family N-acetyltransferase	-
EZZ25_RS16045	390959..391216	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EZZ25_RS16050	391204..391506	+	303	WP_000229316.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EZZ25_RS16060	391742..392659	+	918	WP_000186328.1	alpha/beta hydrolase	-
EZZ25_RS16065	392867..393109	+	243	WP_165490568.1	hypothetical protein	-
EZZ25_RS16070	393284..393673	+	390	WP_001081302.1	hypothetical protein	-
EZZ25_RS16075	393809..394234	+	426	WP_002045007.1	GNAT family N-acetyltransferase	-
EZZ25_RS16080	394409..394936	+	528	Protein_402	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	391646..423467	31821
	-	inside	Integron	-	-	322986..403614	80628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-69)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11753.71 Da
• Isoelectric Point: 5.1972

>T119904 WP_000229316.1 NZ_CP036500:391204-391506 [Vibrio cholerae]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVNPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Nucleotide

• Length: 303 bp

>T119904 NZ_CP036500:391204-391506 [Vibrio cholerae]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAATCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9647.15 Da
• Isoelectric Point: 7.3178

>AT119904 WP_000861987.1 NZ_CP036500:390959-391216 [Vibrio cholerae]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Nucleotide

• Length: 258 bp

>AT119904 NZ_CP036500:390959-391216 [Vibrio cholerae]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1T4SLM9