Detailed information of TA system TA119903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 389802..390757
• Replicon: chromosome
• Accession: NZ_CP036500
• Organism: Vibrio cholerae strain 20000

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: EZZ25_RS16035
• Protein ID: WP_000118351.1
• Coordinates: 390224..390757 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EZZ25_RS16030
• Protein ID: WP_001882332.1
• Coordinates: 389802..390227 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ25_RS19355	384819..385322	+	504	WP_001898452.1	hypothetical protein	-
EZZ25_RS15970	385550..385807	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EZZ25_RS15975	385795..386097	+	303	WP_000229325.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EZZ25_RS15985	386482..386592	+	111	WP_076025930.1	DUF645 family protein	-
EZZ25_RS15990	386922..387146	+	225	WP_161571222.1	DUF3709 domain-containing protein	-
EZZ25_RS19435	387347..387508	+	162	Protein_387	acetyltransferase	-
EZZ25_RS19440	387594..387765	+	172	Protein_388	DUF645 family protein	-
EZZ25_RS16000	387916..388584	+	669	WP_002033340.1	hypothetical protein	-
EZZ25_RS16005	388581..388697	+	117	WP_088124786.1	DUF3265 domain-containing protein	-
EZZ25_RS19445	388803..388838	+	36	WP_172884631.1	hypothetical protein	-
EZZ25_RS16015	388856..389116	+	261	WP_032468027.1	DUF3709 domain-containing protein	-
EZZ25_RS16025	389364..389735	+	372	WP_001164078.1	nucleotide pyrophosphohydrolase	-
EZZ25_RS16030	389802..390227	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
EZZ25_RS16035	390224..390757	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
EZZ25_RS16045	390959..391216	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EZZ25_RS16050	391204..391506	+	303	WP_000229316.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EZZ25_RS16060	391742..392659	+	918	WP_000186328.1	alpha/beta hydrolase	-
EZZ25_RS16065	392867..393109	+	243	WP_165490568.1	hypothetical protein	-
EZZ25_RS16070	393284..393673	+	390	WP_001081302.1	hypothetical protein	-
EZZ25_RS16075	393809..394234	+	426	WP_002045007.1	GNAT family N-acetyltransferase	-
EZZ25_RS16080	394409..394936	+	528	Protein_402	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	391646..423467	31821
	-	inside	Integron	-	-	322986..403614	80628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T119903 WP_000118351.1 NZ_CP036500:390224-390757 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T119903 NZ_CP036500:390224-390757 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT119903 WP_001882332.1 NZ_CP036500:389802-390227 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT119903 NZ_CP036500:389802-390227 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94