Detailed information of TA system TA119899

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 358605..359133
• Replicon: chromosome
• Accession: NZ_CP036500
• Organism: Vibrio cholerae strain 20000

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A0X1KZS6
• Locus tag: EZZ25_RS15670
• Protein ID: WP_000221354.1
• Coordinates: 358846..359133 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A0X1KZS8
• Locus tag: EZZ25_RS15665
• Protein ID: WP_001250179.1
• Coordinates: 358605..358856 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ25_RS15610	353861..354043	+	183	WP_088124806.1	DUF645 family protein	-
EZZ25_RS15615	354443..354577	+	135	WP_080006746.1	DUF645 family protein	-
EZZ25_RS15625	354933..355112	+	180	WP_029628718.1	DUF645 family protein	-
EZZ25_RS15630	355316..355804	+	489	WP_000424242.1	hypothetical protein	-
EZZ25_RS15635	355941..356582	+	642	WP_000582578.1	hypothetical protein	-
EZZ25_RS15640	356603..356695	+	93	WP_001905735.1	DUF3265 domain-containing protein	-
EZZ25_RS15655	357212..357709	+	498	WP_000259905.1	GNAT family N-acetyltransferase	-
EZZ25_RS15660	357862..358401	+	540	WP_001898634.1	phosphoglycerate mutase family protein	-
EZZ25_RS15665	358605..358856	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EZZ25_RS15670	358846..359133	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EZZ25_RS15675	359279..359878	+	600	WP_057558148.1	glutathione S-transferase N-terminal domain-containing protein	-
EZZ25_RS15680	360201..360353	+	153	WP_001884520.1	DUF645 family protein	-
EZZ25_RS15690	360503..361084	+	582	WP_043990376.1	nucleotidyltransferase family protein	-
EZZ25_RS15700	361381..361560	+	180	WP_025998961.1	DUF645 family protein	-
EZZ25_RS15705	361913..362094	+	182	Protein_342	DUF645 family protein	-
EZZ25_RS19420	362504..362600	+	97	Protein_343	DUF645 family protein	-
EZZ25_RS15730	362816..363211	+	396	WP_000870864.1	DUF3465 domain-containing protein	-
EZZ25_RS15735	363517..363698	+	182	Protein_345	DUF645 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	322986..403614	80628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (11-71)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10991.96 Da
• Isoelectric Point: 10.4934

>T119899 WP_000221354.1 NZ_CP036500:358846-359133 [Vibrio cholerae]
MTYKLKFLPAAQKEWSKLAPTIQSQFKKKLKERLENPHVPSAKLRGYDAVYKIKLRTAGYRLAYEVIDDEIVVYVLAVGK
RDKDAVYKKLASRFG

Nucleotide

• Length: 288 bp

>T119899 NZ_CP036500:358846-359133 [Vibrio cholerae]
ATGACCTATAAGTTAAAGTTTCTGCCGGCTGCGCAAAAGGAATGGAGTAAGTTAGCTCCGACAATTCAGAGTCAGTTCAA
GAAGAAGTTAAAGGAACGATTAGAAAATCCGCACGTCCCTTCGGCTAAGCTTCGAGGATACGACGCTGTTTACAAGATTA
AACTTCGTACGGCGGGTTATCGTTTAGCTTATGAGGTTATTGACGATGAGATAGTTGTCTATGTCCTTGCTGTCGGTAAA
CGTGACAAAGATGCTGTCTATAAAAAACTGGCTTCACGCTTCGGTTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9136.29 Da
• Isoelectric Point: 4.0197

>AT119899 WP_001250179.1 NZ_CP036500:358605-358856 [Vibrio cholerae]
MRQVLANCSASISELKKNPTALLNEADGSAIAILNHNKPAAYLVPAETYEYLIDMLDDYELSQIVDSRRADLAQAVEVNI
DDL

Nucleotide

• Length: 252 bp

>AT119899 NZ_CP036500:358605-358856 [Vibrio cholerae]
ATGAGACAAGTTTTAGCGAATTGCTCTGCAAGTATTTCAGAGTTAAAGAAGAACCCAACAGCTTTGTTGAATGAGGCTGA
TGGGTCTGCAATTGCAATTTTAAACCACAACAAGCCAGCGGCTTACCTTGTGCCAGCGGAAACGTATGAGTATCTCATCG
ATATGCTCGATGATTATGAGCTTTCCCAAATTGTCGATAGTCGCCGAGCTGACTTAGCACAAGCTGTGGAAGTAAATATT
GATGACCTATAA

Structures

Toxin

• AlphaFold DB: A0A0X1KZS6

Antitoxin

• AlphaFold DB: A0A0X1KZS8