Detailed information of TA system TA119898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 339777..340315
• Replicon: chromosome
• Accession: NZ_CP036500
• Organism: Vibrio cholerae strain 20000

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q9KMJ0
• Locus tag: EZZ25_RS15475
• Protein ID: WP_000802136.1
• Coordinates: 339777..340076 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A271VK51
• Locus tag: EZZ25_RS15480
• Protein ID: WP_001107720.1
• Coordinates: 340073..340315 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ25_RS15420	335001..335471	+	471	WP_131476975.1	hypothetical protein	-
EZZ25_RS19325	335481..335624	+	144	WP_155648918.1	hypothetical protein	-
EZZ25_RS15425	335784..336227	+	444	WP_000434669.1	hypothetical protein	-
EZZ25_RS15440	336524..336703	+	180	WP_088124781.1	DUF645 family protein	-
EZZ25_RS15445	337058..337239	+	182	Protein_303	DUF645 family protein	-
EZZ25_RS19330	337666..337776	+	111	WP_080003861.1	DUF645 family protein	-
EZZ25_RS15460	337980..338477	+	498	WP_000259913.1	GNAT family N-acetyltransferase	-
EZZ25_RS15465	338619..339053	+	435	WP_000453958.1	GNAT family N-acetyltransferase	-
EZZ25_RS15470	339257..339646	+	390	WP_000491258.1	VOC family protein	-
EZZ25_RS15475	339777..340076	-	300	WP_000802136.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EZZ25_RS15480	340073..340315	-	243	WP_001107720.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EZZ25_RS15485	340534..341514	+	981	WP_088124776.1	DUF3883 domain-containing protein	-
EZZ25_RS15495	341806..341987	+	182	Protein_311	DUF645 family protein	-
EZZ25_RS15500	342140..342502	+	363	WP_046122431.1	DUF4144 domain-containing protein	-
EZZ25_RS15505	342689..342973	+	285	WP_000048760.1	antibiotic biosynthesis monooxygenase	-
EZZ25_RS15510	343132..343683	+	552	WP_000821931.1	hypothetical protein	-
EZZ25_RS15515	343846..344262	+	417	WP_000759007.1	DUF1311 domain-containing protein	-
EZZ25_RS15525	344418..344900	+	483	WP_000422942.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	322986..403614	80628

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11589.29 Da
• Isoelectric Point: 9.9232

>T119898 WP_000802136.1 NZ_CP036500:c340076-339777 [Vibrio cholerae]
MKPFNLTVAAKADLRDIALFTQRRWGKEQRNVYLKQFDDSFWLLAENPDIGKSCDEIREGYRKFPQGSHVIFYQQTGSQQ
IRVIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T119898 NZ_CP036500:c340076-339777 [Vibrio cholerae]
ATGAAACCATTTAATCTTACTGTCGCCGCTAAAGCCGATTTACGGGATATTGCTTTATTCACTCAACGACGCTGGGGAAA
AGAGCAGCGAAATGTTTATTTAAAGCAATTCGATGACTCATTTTGGCTTTTAGCGGAAAATCCCGACATCGGTAAATCAT
GCGATGAAATCCGAGAGGGATACAGAAAATTTCCCCAAGGGAGTCACGTCATTTTTTATCAGCAAACCGGCAGCCAACAA
ATCAGGGTCATCCGAATTCTTCATAAAAGCATGGATGTGAACCCAATATTCGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.82 Da
• Isoelectric Point: 4.1677

>AT119898 WP_001107720.1 NZ_CP036500:c340315-340073 [Vibrio cholerae]
MAKNTSITLGEHFDGFITSQIQSGRYGSASEVIRSALRLLENQETKLQSLRQLLVEGEQSGDADYDLDSFINELDSENIR

Nucleotide

• Length: 243 bp

>AT119898 NZ_CP036500:c340315-340073 [Vibrio cholerae]
ATGGCTAAAAACACAAGTATCACTCTTGGTGAACACTTCGATGGCTTTATTACAAGCCAAATACAAAGTGGACGTTACGG
CTCAGCAAGTGAAGTAATTCGCTCTGCGCTACGTCTACTCGAAAACCAAGAAACCAAACTACAGTCACTCCGTCAATTAC
TTGTTGAAGGAGAGCAAAGTGGTGACGCTGATTATGACCTTGATAGTTTCATCAATGAACTCGATAGTGAAAACATTCGA
TGA

Structures

Toxin

• PDB: 7R5A

Antitoxin

• AlphaFold DB: A0A271VK51