Detailed information of TA system TA119886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1518174..1518812
• Replicon: chromosome
• Accession: NZ_CP036496
• Organism: Paenibacillus polymyxa strain KN-03

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: ERJ71_RS06610
• Protein ID: WP_007429335.1
• Coordinates: 1518462..1518812 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H6CFY0
• Locus tag: ERJ71_RS06605
• Protein ID: WP_007429334.1
• Coordinates: 1518174..1518455 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ERJ71_RS06590 (ERJ71_06520)	1514395..1515027	+	633	WP_019686557.1	LysE family transporter	-
ERJ71_RS06595 (ERJ71_06525)	1515138..1516415	+	1278	WP_019686558.1	outer membrane lipoprotein-sorting protein	-
ERJ71_RS06600 (ERJ71_06530)	1516684..1517880	+	1197	WP_029514916.1	alanine racemase	-
ERJ71_RS06605 (ERJ71_06535)	1518174..1518455	+	282	WP_007429334.1	ribbon-helix-helix protein, CopG family	Antitoxin
ERJ71_RS06610 (ERJ71_06540)	1518462..1518812	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ERJ71_RS06615 (ERJ71_06545)	1518991..1521213	+	2223	WP_029514917.1	Tex family protein	-
ERJ71_RS06620 (ERJ71_06550)	1521317..1521451	-	135	WP_016821163.1	cortex morphogenetic protein CmpA	-
ERJ71_RS06625 (ERJ71_06555)	1521594..1522106	+	513	WP_016821162.1	SprT family protein	-
ERJ71_RS06640 (ERJ71_06570)	1522485..1523360	-	876	WP_019686561.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	groEL	1367933..1526424	158491

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T119886 WP_007429335.1 NZ_CP036496:1518462-1518812 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T119886 NZ_CP036496:1518462-1518812 [Paenibacillus polymyxa]
TTGATCGTAAAGCGCGGCGACGTTTTTTTTGCAGACCTTTCACCCGTAGTCGGTTCCGAGCAAGGTGGCGTCAGACCTGT
GCTGATCATTCAAAATGATATCGGCAACCGGTTTAGCCCTACCGTGATCGTAGCAGCCATCACTGCACAGATTCAGAAGG
CAAAGCTGCCTACACACGTGGAAATCGACGCGGCGACGCATGGCTTTGATCGTGATTCTGTTGTTCTTCTGGAGCAAATC
CGGACAATCGACAAGCAAAGGCTTACCGACAAAATCACCCATTTGGATGAAGAAACCATGCGCAAAGTGAGCGACTCGCT
ACAGATCAGTCTCGGTTTGATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10636.25 Da
• Isoelectric Point: 8.5257

>AT119886 WP_007429334.1 NZ_CP036496:1518174-1518455 [Paenibacillus polymyxa]
MANLQNTKRIMISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEE
DANSTLGRTVSGV

Nucleotide

• Length: 282 bp

>AT119886 NZ_CP036496:1518174-1518455 [Paenibacillus polymyxa]
TTGGCCAATTTGCAGAACACCAAAAGAATAATGATCAGTTTGCCAGATCATCTCTTGCAGGAAGTGGACGGGATCGTAGC
TATGGAAAATTCCAACCGCAGTGAATTTATCAGGCAGGCAATGAAGCTTTATGTAGGTGAACGTAAAAAACGTTACATTC
GTGAAGCTATGCAGCGTGGATACATGGAAATGGCTAAAATTAACCTGACCATGGCATCCGAAGCCTTTCACGCGGAGGAA
GATGCAAACAGCACCTTGGGCCGTACAGTTAGCGGGGTGTAA

Structures

Toxin

• AlphaFold DB: H6CFY1

Antitoxin

• AlphaFold DB: H6CFY0