Detailed information of TA system TA119885

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 5533173..5534052
• Replicon: chromosome
• Accession: NZ_CP036495
• Organism: Pseudomonas viridiflava strain Psy35

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EZZ81_RS24600
• Protein ID: WP_029243149.1
• Coordinates: 5533699..5534052 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A5E6PJ22
• Locus tag: EZZ81_RS24595
• Protein ID: WP_029243148.1
• Coordinates: 5533173..5533646 (-)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ81_RS24580 (EZZ81_24955)	5529589..5530446	+	858	WP_029243146.1	poly(3-hydroxyalkanoate) depolymerase	-
EZZ81_RS24585 (EZZ81_24960)	5530611..5532296	+	1686	WP_029243147.1	class II poly(R)-hydroxyalkanoic acid synthase	-
EZZ81_RS24590 (EZZ81_24965)	5532377..5532991	+	615	WP_004882680.1	TetR/AcrR family transcriptional regulator	-
EZZ81_RS24595 (EZZ81_24970)	5533173..5533646	-	474	WP_029243148.1	transcriptional regulator	Antitoxin
EZZ81_RS24600 (EZZ81_24975)	5533699..5534052	-	354	WP_029243149.1	type II toxin-antitoxin system HigB family toxin	Toxin
EZZ81_RS24605 (EZZ81_24980)	5534231..5535016	-	786	WP_029243150.1	phasin family protein	-
EZZ81_RS24610 (EZZ81_24985)	5535027..5535500	-	474	WP_029243151.1	phasin family protein	-
EZZ81_RS24615 (EZZ81_24990)	5535661..5535939	-	279	WP_004882685.1	polyhydroxyalkanoic acid system family protein	-
EZZ81_RS24620 (EZZ81_24995)	5536084..5536854	+	771	WP_004882686.1	bifunctional demethylmenaquinone methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol methylase UbiE	-
EZZ81_RS24625 (EZZ81_25000)	5536854..5537477	+	624	WP_029243152.1	SCP2 sterol-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13538.75 Da
• Isoelectric Point: 10.0467

>T119885 WP_029243149.1 NZ_CP036495:c5534052-5533699 [Pseudomonas viridiflava]
MRVIAKTTVAKAIEAHGQWKAALSLWLTTFDRATLRFESFEQLRDIWVTASGWNIDRIPCSKLRQPSVKGPLDVYVFDIK
KNECRIIAWINTRNGTIYIKDILPHAGYDKWCKSDVK

Nucleotide

• Length: 354 bp

>T119885 NZ_CP036495:c5534052-5533699 [Pseudomonas viridiflava]
ATGCGGGTAATAGCAAAAACGACGGTTGCGAAAGCCATCGAAGCTCATGGTCAGTGGAAGGCGGCACTGAGTTTGTGGCT
GACCACCTTTGACAGGGCGACTCTGCGCTTTGAGTCCTTCGAGCAGCTTCGAGATATCTGGGTCACCGCTTCTGGATGGA
ACATTGACCGAATTCCCTGCTCGAAGCTGAGACAGCCGAGCGTTAAAGGGCCTCTTGATGTGTATGTCTTCGACATCAAG
AAAAATGAGTGCCGGATTATTGCGTGGATTAATACCCGGAATGGCACTATTTATATAAAGGATATTCTGCCCCACGCCGG
TTATGACAAGTGGTGCAAGAGTGACGTTAAGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17239.59 Da
• Isoelectric Point: 4.8200

>AT119885 WP_029243148.1 NZ_CP036495:c5533646-5533173 [Pseudomonas viridiflava]
MKHDKNGATGLDSFIANISPMLDGLSDQIATMAQLLAACHDPVKSDTDLKGRMALIDELYSHADSEDHAAARFAELVAER
VYEYETETVLIPYSSQAEALAFLISDRGVRQKDLSGVATQSAVSEILNNKRKMTVAQIKGFAEFFKVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT119885 NZ_CP036495:c5533646-5533173 [Pseudomonas viridiflava]
ATGAAGCATGATAAGAACGGAGCCACTGGGCTGGATTCCTTCATCGCTAACATTTCACCGATGCTTGATGGCTTAAGCGA
TCAGATAGCAACGATGGCCCAGTTATTGGCTGCCTGTCATGATCCCGTCAAGAGTGACACTGACTTGAAGGGGCGGATGG
CGCTGATCGATGAGCTTTACTCTCATGCAGACAGTGAAGATCACGCAGCCGCGCGCTTTGCGGAGCTGGTGGCCGAGCGT
GTGTATGAGTACGAGACCGAAACCGTTTTGATTCCTTACTCATCTCAGGCCGAAGCACTGGCTTTCCTGATTTCGGACAG
GGGAGTCAGGCAGAAGGATTTGTCAGGTGTTGCCACTCAAAGTGCTGTTTCCGAGATACTGAACAACAAGAGGAAAATGA
CGGTTGCTCAGATAAAAGGCTTTGCAGAATTTTTTAAAGTGCCCGTCGAGTTTTTCATGCATGGCGTCGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E6PJ22