Detailed information of TA system TA119882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 1288267..1288965
• Replicon: chromosome
• Accession: NZ_CP036495
• Organism: Pseudomonas viridiflava strain Psy35

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EZZ81_RS06080
• Protein ID: WP_004881256.1
• Coordinates: 1288783..1288965 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A5E6SCC0
• Locus tag: EZZ81_RS06075
• Protein ID: WP_025996829.1
• Coordinates: 1288267..1288677 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ81_RS06055 (EZZ81_06140)	1284046..1285215	+	1170	WP_004881265.1	mandelate racemase/muconate lactonizing enzyme family protein	-
EZZ81_RS06060 (EZZ81_06145)	1285293..1286609	+	1317	WP_029243206.1	C4-dicarboxylate transporter DctA	-
EZZ81_RS06065 (EZZ81_06150)	1286660..1286971	-	312	WP_004881262.1	helix-turn-helix domain-containing protein	-
EZZ81_RS06070 (EZZ81_06155)	1287049..1288140	+	1092	WP_029243205.1	NADH:flavin oxidoreductase/NADH oxidase	-
EZZ81_RS06075 (EZZ81_06160)	1288267..1288677	-	411	WP_025996829.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EZZ81_RS06080 (EZZ81_06165)	1288783..1288965	-	183	WP_004881256.1	type II toxin-antitoxin system HicA family toxin	Toxin
EZZ81_RS06085 (EZZ81_06170)	1289251..1292307	-	3057	WP_080665252.1	autotransporter domain-containing protein	-
EZZ81_RS06090 (EZZ81_06175)	1292594..1293094	+	501	WP_029243203.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6888.95 Da
• Isoelectric Point: 10.5417

>T119882 WP_004881256.1 NZ_CP036495:c1288965-1288783 [Pseudomonas viridiflava]
MRSREMIRTIEDDGWYLVATKGSHHQYKHSSKPGRVTIKHPDSDIPIKTMNSILKQAGLK

Nucleotide

• Length: 183 bp

>T119882 NZ_CP036495:c1288965-1288783 [Pseudomonas viridiflava]
ATGCGAAGCAGGGAAATGATAAGAACGATTGAGGATGATGGATGGTATCTCGTGGCGACCAAAGGTAGTCACCATCAATA
CAAGCACTCGTCAAAGCCTGGAAGGGTAACGATCAAGCACCCGGATTCCGATATACCGATCAAAACGATGAATAGCATAT
TGAAGCAAGCGGGATTGAAATGA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 14851.63 Da
• Isoelectric Point: 4.6312

>AT119882 WP_025996829.1 NZ_CP036495:c1288677-1288267 [Pseudomonas viridiflava]
MKFTVVLHKDADSDYGVTVPDVPGCFSAGSSVSEALESVKEALSLHFEGLVADGAPLPNAQAIDIHMANPDYEDGIWAVV
EFDVTPYFGKSVRFNATLPEHLLAKIDERVKTDHRYASRSGFLAAAALNELHTEYR

Nucleotide

• Length: 411 bp

>AT119882 NZ_CP036495:c1288677-1288267 [Pseudomonas viridiflava]
ATGAAATTCACCGTCGTTCTGCATAAAGACGCCGATTCAGACTATGGAGTAACTGTTCCGGACGTGCCTGGTTGCTTTTC
GGCAGGCAGCTCGGTATCGGAAGCACTGGAAAGTGTGAAGGAGGCGTTATCGCTGCACTTTGAAGGTCTGGTGGCCGATG
GCGCACCACTCCCCAATGCGCAAGCCATTGATATTCATATGGCCAATCCCGACTACGAAGACGGTATCTGGGCGGTGGTC
GAGTTCGACGTGACGCCTTATTTTGGCAAGTCAGTACGATTCAACGCGACACTTCCGGAACACCTGCTGGCCAAAATTGA
TGAGCGCGTCAAAACCGATCATCGATACGCCTCACGCTCTGGCTTTTTGGCCGCTGCGGCATTGAACGAGCTGCATACTG
AGTACCGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E6SCC0