Detailed information of TA system TA119875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/RHH(antitoxin)
• Location: 3349483..3350036
• Replicon: chromosome
• Accession: NZ_CP036494
• Organism: Pseudomonas putida strain PP1

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q88JZ3
• Locus tag: EZZ80_RS15400
• Protein ID: WP_010953434.1
• Coordinates: 3349483..3349776 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: Q88JZ4
• Locus tag: EZZ80_RS15405
• Protein ID: WP_010953433.1
• Coordinates: 3349764..3350036 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EZZ80_RS15370 (EZZ80_15665)	3344637..3345155	-	519	WP_049587643.1	DUF1993 domain-containing protein	-
EZZ80_RS15375	3345298..3345510	-	213	WP_061405625.1	hypothetical protein	-
EZZ80_RS15380 (EZZ80_15675)	3345507..3345716	-	210	WP_010953438.1	4-oxalocrotonate tautomerase family protein	-
EZZ80_RS15385 (EZZ80_15680)	3345786..3346517	-	732	WP_010953437.1	SDR family NAD(P)-dependent oxidoreductase	-
EZZ80_RS15390 (EZZ80_15685)	3346631..3347557	+	927	WP_010953436.1	LysR family transcriptional regulator	-
EZZ80_RS15395 (EZZ80_15690)	3348080..3349489	+	1410	WP_010953435.1	site-specific integrase	-
EZZ80_RS15400 (EZZ80_15695)	3349483..3349776	-	294	WP_010953434.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EZZ80_RS15405 (EZZ80_15700)	3349764..3350036	-	273	WP_010953433.1	CopG family ribbon-helix-helix protein	Antitoxin
EZZ80_RS15410 (EZZ80_15705)	3350161..3350406	-	246	WP_010953432.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EZZ80_RS15415 (EZZ80_15710)	3350611..3350940	+	330	WP_010953431.1	hypothetical protein	-
EZZ80_RS15420 (EZZ80_15715)	3351038..3352006	+	969	WP_031299399.1	DUF932 domain-containing protein	-
EZZ80_RS15425 (EZZ80_15720)	3352086..3353090	+	1005	WP_010953429.1	YqaJ viral recombinase family protein	-
EZZ80_RS15430 (EZZ80_15725)	3353183..3354133	+	951	WP_031299400.1	hypothetical protein	-
EZZ80_RS15435 (EZZ80_15730)	3354105..3354464	+	360	Protein_3064	DNA repair protein RadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3336998..3380476	43478

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-95)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11155.61 Da
• Isoelectric Point: 5.9081

>T119875 WP_010953434.1 NZ_CP036494:c3349776-3349483 [Pseudomonas putida]
VPRLIVTEGAAKGLERCRRFLSDKDPQVARRAAQAIERQFARLEESPEVGRPFPDLPELRELIIEFGDSGYVALYRYERA
DDTAYVLAFRHQKEAGY

Nucleotide

• Length: 294 bp

>T119875 NZ_CP036494:c3349776-3349483 [Pseudomonas putida]
GTGCCACGACTGATCGTCACTGAAGGCGCGGCGAAGGGCCTGGAGCGTTGCCGGCGATTTCTCTCCGATAAAGATCCGCA
GGTCGCACGCCGTGCTGCACAGGCAATCGAGCGCCAATTCGCTCGATTGGAGGAAAGTCCGGAAGTAGGGCGTCCTTTTC
CAGATCTGCCAGAGCTGCGTGAGCTCATCATCGAGTTTGGTGACTCAGGTTACGTCGCGCTGTACCGCTATGAGCGCGCA
GATGACACCGCCTATGTCTTGGCCTTTCGCCATCAAAAAGAAGCTGGCTACTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10373.53 Da
• Isoelectric Point: 5.5744

>AT119875 WP_010953433.1 NZ_CP036494:c3350036-3349764 [Pseudomonas putida]
MATSIKIDDDLKGRIQHLACLRQRSSHWIMREAITQYVEREEARESFKQEALASWAAYQETGQHLTGQEARTWLGSWGTD
AEAELPKCHD

Nucleotide

• Length: 273 bp

>AT119875 NZ_CP036494:c3350036-3349764 [Pseudomonas putida]
ATGGCCACTTCCATCAAAATTGACGACGATCTGAAAGGTCGCATTCAGCATTTGGCTTGTTTGCGCCAGCGCTCATCTCA
CTGGATCATGCGTGAGGCGATCACCCAGTACGTCGAGCGTGAGGAGGCCCGTGAGAGCTTCAAACAAGAAGCGCTGGCAT
CTTGGGCTGCATACCAGGAAACCGGCCAGCACTTGACCGGCCAGGAGGCTCGCACCTGGCTTGGTAGTTGGGGTACAGAT
GCTGAGGCAGAGTTACCCAAGTGCCACGACTGA

Structures

Toxin

• AlphaFold DB: A0A2W5CNE8

Antitoxin

• AlphaFold DB: Q88JZ4