Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 1558661..1559269 Replicon chromosome
Accession NZ_CP036492
Organism Pseudomonas aeruginosa strain Paer4

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag EZZ78_RS07125 Protein ID WP_003114156.1
Coordinates 1558922..1559269 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag EZZ78_RS07120 Protein ID WP_003114155.1
Coordinates 1558661..1558912 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EZZ78_RS07100 (EZZ78_07245) 1554308..1554664 + 357 WP_003114150.1 DUF2523 family protein -
EZZ78_RS07105 (EZZ78_07250) 1554668..1555942 + 1275 WP_010895520.1 zonular occludens toxin family protein -
EZZ78_RS07110 (EZZ78_07255) 1556172..1557464 + 1293 WP_003115206.1 hypothetical protein -
EZZ78_RS07115 (EZZ78_07260) 1557464..1558447 + 984 WP_003114154.1 tyrosine-type recombinase/integrase -
EZZ78_RS07120 (EZZ78_07265) 1558661..1558912 + 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EZZ78_RS07125 (EZZ78_07270) 1558922..1559269 + 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EZZ78_RS07135 (EZZ78_07280) 1559596..1560498 - 903 WP_003114157.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
EZZ78_RS07140 (EZZ78_07285) 1560950..1561687 - 738 WP_003114158.1 murein L,D-transpeptidase catalytic domain family protein -
EZZ78_RS07145 (EZZ78_07290) 1561767..1562810 - 1044 WP_003098363.1 L,D-transpeptidase -
EZZ78_RS07150 (EZZ78_07295) 1562946..1563638 - 693 WP_003114159.1 16S rRNA pseudouridine(516) synthase -
EZZ78_RS07155 (EZZ78_07300) 1563638..1563910 - 273 WP_010895521.1 cysteine-rich CWC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage catB7 - 1537092..1568373 31281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T119862 WP_003114156.1 NZ_CP036492:1558922-1559269 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T119862 NZ_CP036492:1558922-1559269 [Pseudomonas aeruginosa]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT119862 WP_003114155.1 NZ_CP036492:1558661-1558912 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT119862 NZ_CP036492:1558661-1558912 [Pseudomonas aeruginosa]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References