Detailed information of TA system TA119859

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1642611..1643212
• Replicon: chromosome
• Accession: NZ_CP036487
• Organism: Thermoactinomyces vulgaris strain CDF

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: AB849_RS08460
• Protein ID: WP_037994646.1
• Coordinates: 1642611..1642940 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A151YYH9
• Locus tag: AB849_RS08465
• Protein ID: WP_037994644.1
• Coordinates: 1642940..1643212 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB849_RS08455	1640417..1642129	-	1713	WP_049720374.1	hypothetical protein	-
AB849_RS13320	1642216..1642389	-	174	WP_156097721.1	hypothetical protein	-
AB849_RS08460	1642611..1642940	-	330	WP_037994646.1	endoribonuclease MazF	Toxin
AB849_RS08465	1642940..1643212	-	273	WP_037994644.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
AB849_RS08470	1643414..1644118	+	705	WP_037994643.1	YdcF family protein	-
AB849_RS08475	1644110..1644775	-	666	WP_049720373.1	hypothetical protein	-
AB849_RS08480	1645202..1646611	+	1410	WP_049720372.1	cytochrome ubiquinol oxidase subunit I	-
AB849_RS08485	1646595..1647611	+	1017	WP_037994635.1	cytochrome d ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-98)

Antitoxin

• SpoVT-AbrB domain (18-62)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12112.01 Da
• Isoelectric Point: 8.5259

>T119859 WP_037994646.1 NZ_CP036487:c1642940-1642611 [Thermoactinomyces vulgaris]
MAVPDRGDLVYVSFHPQAGHEQAGRRPAIVLSPQSFNAVTKFAVVCPITRKEKGYPFEVKLPDGLPIQGVILADQVKSLD
WRARQFTIKGRAPENVVNDCIELIHTFLS

Nucleotide

• Length: 330 bp

>T119859 NZ_CP036487:c1642940-1642611 [Thermoactinomyces vulgaris]
ATGGCTGTACCGGATCGCGGGGATCTTGTTTATGTCAGTTTCCATCCACAAGCCGGGCATGAACAGGCCGGAAGACGCCC
CGCCATTGTCTTATCCCCCCAATCATTCAACGCAGTGACCAAATTCGCTGTGGTCTGCCCCATCACCCGCAAAGAAAAAG
GCTACCCATTTGAAGTGAAATTGCCCGACGGTCTGCCCATTCAAGGCGTCATTCTGGCGGACCAAGTCAAAAGCCTGGAT
TGGCGGGCACGGCAATTTACGATCAAAGGCCGGGCACCCGAAAACGTGGTTAATGATTGCATTGAATTAATCCATACCTT
TTTGTCATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10264.92 Da
• Isoelectric Point: 6.4781

>AT119859 WP_037994644.1 NZ_CP036487:c1643212-1642940 [Thermoactinomyces vulgaris]
MKEEENQGGIMKMTTQVRKWGNSLAIRIPSHIAEMLSIKQGSELEMKVENQEIKLVPKKKKPTLDELLAKVTPENRHEEI
QLGTEGNELI

Nucleotide

• Length: 273 bp

>AT119859 NZ_CP036487:c1643212-1642940 [Thermoactinomyces vulgaris]
ATGAAAGAGGAGGAAAATCAAGGAGGAATCATGAAGATGACGACACAAGTCCGTAAATGGGGAAACAGCCTGGCCATTCG
GATCCCCAGCCATATTGCCGAGATGTTGTCCATCAAGCAAGGCTCCGAATTGGAAATGAAAGTGGAAAACCAAGAGATTA
AATTGGTTCCAAAGAAGAAAAAACCCACATTGGATGAACTTCTAGCCAAAGTCACGCCGGAAAACCGCCACGAGGAAATT
CAGCTTGGAACCGAAGGGAATGAGCTGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A151YYH9