Detailed information of TA system TA119858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 681292..681899
• Replicon: chromosome
• Accession: NZ_CP036487
• Organism: Thermoactinomyces vulgaris strain CDF

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A151YVK1
• Locus tag: AB849_RS03475
• Protein ID: WP_037998060.1
• Coordinates: 681292..681642 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: AB849_RS03480
• Protein ID: WP_175413226.1
• Coordinates: 681648..681899 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AB849_RS03450	676939..678219	+	1281	WP_037998052.1	Glu/Leu/Phe/Val dehydrogenase	-
AB849_RS03455	678392..679177	-	786	WP_062843382.1	RNA polymerase sigma factor SigB	-
AB849_RS03460	679140..679628	-	489	WP_037998054.1	anti-sigma B factor RsbW	-
AB849_RS03465	679625..679960	-	336	WP_037998057.1	STAS domain-containing protein	-
AB849_RS03470	679957..681018	-	1062	WP_052186901.1	PP2C family protein-serine/threonine phosphatase	-
AB849_RS03475	681292..681642	-	351	WP_037998060.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
AB849_RS03480	681648..681899	-	252	WP_175413226.1	ribbon-helix-helix protein, CopG family	Antitoxin
AB849_RS03485	682123..683304	-	1182	WP_037998078.1	alanine racemase	-
AB849_RS03490	683324..683821	-	498	WP_131470884.1	hypothetical protein	-
AB849_RS03495	683862..684842	-	981	WP_131470885.1	outer membrane lipoprotein carrier protein LolA	-
AB849_RS03500	684938..686476	-	1539	WP_121874496.1	NAD(P)H-hydrate dehydratase	-
AB849_RS03505	686519..686899	-	381	WP_131470886.1	holo-ACP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-35)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12939.02 Da
• Isoelectric Point: 5.6976

>T119858 WP_037998060.1 NZ_CP036487:c681642-681292 [Thermoactinomyces vulgaris]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKAYGFDRDSVILLEQI
RTIDKQRLTDKITHLDDEMMNRVNEALMISLGMINF

Nucleotide

• Length: 351 bp

>T119858 NZ_CP036487:c681642-681292 [Thermoactinomyces vulgaris]
TTGATTGTGAAACGTGGCGATGTTTATTTTGCTGATCTATCCCCTGTGGTCGGATCCGAACAAGGTGGGGTTCGGCCTGT
TTTGGTGATTCAAAATGATATTGGCAATCGGTTCAGCCCCACCGTGATCGTCGCCGCGATTACAGCTCAAATTCAAAAGG
CCAAATTACCTACGCATGTGGAAATCGATGCCAAAGCATACGGGTTTGATCGTGACTCGGTCATATTGTTGGAACAAATC
CGAACGATCGATAAGCAAAGGCTGACGGATAAAATCACTCATCTGGATGATGAAATGATGAATCGTGTCAATGAAGCGTT
AATGATCAGTTTGGGTATGATTAACTTCTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9737.22 Da
• Isoelectric Point: 4.8898

>AT119858 WP_175413226.1 NZ_CP036487:c681899-681648 [Thermoactinomyces vulgaris]
MISLPTKLLQEVDGVVARENSNRSEFIRQAMRMYLQERKKRLIREMMQRGYMEMARINLNIASEAFEAEEEAEDTLDRLV
SGV

Nucleotide

• Length: 252 bp

>AT119858 NZ_CP036487:c681899-681648 [Thermoactinomyces vulgaris]
ATGATCAGCCTGCCAACAAAATTGCTTCAAGAAGTGGATGGCGTTGTGGCGAGGGAAAATTCCAATCGGAGTGAGTTTAT
CCGCCAAGCGATGAGAATGTATCTCCAAGAGCGGAAAAAGCGTCTGATTCGTGAAATGATGCAACGCGGCTACATGGAGA
TGGCACGCATCAATCTCAACATCGCTTCGGAAGCATTTGAGGCAGAAGAAGAGGCTGAAGATACTTTGGATCGATTGGTT
AGTGGGGTGTAA

Structures

Toxin

• AlphaFold DB: A0A151YVK1