Detailed information of TA system TA119855

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 20382..20950
• Replicon: plasmid pER79ag32
• Accession: NZ_CP036466
• Organism: Zymomonas mobilis strain ER79ag

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EWS78_RS09130
• Protein ID: WP_062339523.1
• Coordinates: 20621..20950 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: EWS78_RS09125
• Protein ID: WP_062339521.1
• Coordinates: 20382..20624 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWS78_RS09115	15428..17200	+	1773	WP_185761829.1	helix-turn-helix domain-containing protein	-
EWS78_RS09120	17611..18389	-	779	Protein_14	IS5 family transposase	-
EWS78_RS09125	20382..20624	+	243	WP_062339521.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EWS78_RS09130	20621..20950	+	330	WP_062339523.1	endoribonuclease MazF	Toxin
EWS78_RS09135	21024..21191	-	168	WP_160327977.1	hypothetical protein	-
EWS78_RS09140	21449..22891	+	1443	WP_062339526.1	SIR2 family protein	-
EWS78_RS09145	22901..23341	+	441	WP_062339528.1	TIR domain-containing protein	-
EWS78_RS09150	24626..24775	+	150	WP_160327978.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..32792	32792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (12-107)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 12159.39 Da
• Isoelectric Point: 10.6796

>T119855 WP_062339523.1 NZ_CP036466:20621-20950 [Zymomonas mobilis]
VSVKVTTVWVPVWLEFDPQAGREQAGHRPAVVLSPVSYNAKAGMMLCCPTTTKIKGYPFEVALTGKTASVVLSDQVRSLD
WRVRKTKLKDKVLPKELEAIRQRIRLLVG

Nucleotide

• Length: 330 bp

>T119855 NZ_CP036466:20621-20950 [Zymomonas mobilis]
GTGAGTGTCAAAGTAACGACTGTCTGGGTTCCTGTCTGGCTTGAGTTTGATCCGCAGGCGGGCAGGGAGCAGGCTGGGCA
CCGACCTGCTGTTGTGCTGAGTCCTGTCAGCTATAATGCTAAAGCTGGAATGATGCTGTGCTGTCCGACAACGACCAAGA
TCAAAGGCTATCCCTTTGAAGTGGCTCTGACTGGAAAAACTGCCAGTGTGGTTTTGTCAGATCAGGTCAGAAGTCTGGAT
TGGCGCGTGAGAAAGACAAAGCTTAAAGATAAAGTTTTGCCAAAAGAGCTTGAGGCAATTCGGCAGCGCATTAGATTGCT
GGTCGGGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8771.04 Da
• Isoelectric Point: 4.4488

>AT119855 WP_062339521.1 NZ_CP036466:20382-20624 [Zymomonas mobilis]
MQGVVRKWGNSAAIRLPVGVLEAVGLKIDQAVDVREEDGRIIIEPVRAVPLKLEELVSGITVENRHDEMDFGSSVGCESW

Nucleotide

• Length: 243 bp

>AT119855 NZ_CP036466:20382-20624 [Zymomonas mobilis]
ATGCAGGGCGTTGTCAGGAAATGGGGGAACAGTGCAGCCATTCGTCTTCCTGTCGGTGTGCTTGAAGCCGTCGGTCTGAA
GATCGATCAGGCCGTCGATGTGCGAGAAGAAGATGGGCGGATCATCATAGAGCCAGTCAGAGCAGTTCCTCTCAAACTGG
AAGAATTAGTGTCAGGTATTACGGTCGAGAACCGCCATGATGAAATGGACTTTGGTTCATCTGTTGGTTGTGAAAGCTGG
TGA