Detailed information of TA system TA119848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 2495..3282
• Replicon: plasmid pER79ap33
• Accession: NZ_CP036459
• Organism: Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821 strain ZM4

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: EWM65_RS09190
• Protein ID: WP_062339594.1
• Coordinates: 2788..3282 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: EWM65_RS09185
• Protein ID: WP_062339592.1
• Coordinates: 2495..2791 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EWM65_RS09165	410..646	+	237	WP_062339585.1	hypothetical protein	-
EWM65_RS09170	643..1224	+	582	WP_062339588.1	zeta toxin family protein	-
EWM65_RS09175	1358..1618	-	261	WP_062339589.1	ribbon-helix-helix protein, CopG family	-
EWM65_RS09180	1648..2298	-	651	WP_062339590.1	AAA family ATPase	-
EWM65_RS09185	2495..2791	+	297	WP_062339592.1	DUF1778 domain-containing protein	Antitoxin
EWM65_RS09190	2788..3282	+	495	WP_062339594.1	GNAT family N-acetyltransferase	Toxin
EWM65_RS09195	3635..4540	-	906	WP_062339596.1	LysR family transcriptional regulator	-
EWM65_RS09200	4763..6196	+	1434	WP_108125217.1	carbohydrate porin	-
EWM65_RS09205	6213..7508	+	1296	WP_062339600.1	C4-dicarboxylate transporter DctA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33007	33007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (16-90)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17994.99 Da
• Isoelectric Point: 9.9205

>T119848 WP_062339594.1 NZ_CP036459:2788-3282 [Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821]
VTITAPELVSPEHQVDLFQSGVESLDLWLKKRALKNQMTGASRTFVACKNRRVIAYYALASSAITIKSAHGRFRRNMPDP
IPVVVLGRLAIDQSLHNQGVGRAMVRDAALRIIQAADTIGIRGMLVHALSDEAKSFYEHIGFEPSPIDPMMLMVTLADLK
SSLA

Nucleotide

• Length: 495 bp

>T119848 NZ_CP036459:2788-3282 [Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821]
GTGACAATAACAGCTCCAGAATTAGTATCCCCAGAACATCAAGTTGACTTATTTCAGTCCGGTGTAGAGAGCCTTGATCT
ATGGTTAAAAAAGCGCGCTTTGAAAAATCAAATGACAGGAGCCTCTCGTACCTTTGTAGCCTGTAAAAATCGACGGGTGA
TAGCCTATTATGCTCTAGCATCTAGCGCTATCACTATAAAATCAGCCCATGGACGATTTAGGCGTAATATGCCAGATCCT
ATTCCTGTTGTCGTTTTAGGGCGGCTTGCTATTGATCAATCTTTACATAACCAAGGCGTTGGACGAGCAATGGTAAGGGA
TGCAGCCTTAAGGATAATACAGGCAGCTGATACTATCGGAATTAGAGGTATGCTCGTTCATGCGCTATCTGATGAAGCCA
AATCTTTCTACGAACATATCGGTTTCGAACCGTCTCCAATCGACCCAATGATGTTGATGGTTACATTAGCCGATTTAAAG
TCGAGCCTTGCTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11258.00 Da
• Isoelectric Point: 7.7936

>AT119848 WP_062339592.1 NZ_CP036459:2495-2791 [Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821]
MTAATTIPSPNKREALNIRIKPEERDLIDRAAKIQRKSRTNFVLEAACRAAEEALFDQCLISVDPKAYTDFLMRLDMPPR
PNDRLQKTMQTVAPWDEL

Nucleotide

• Length: 297 bp

>AT119848 NZ_CP036459:2495-2791 [Zymomonas mobilis subsp. mobilis ZM4 = ATCC 31821]
ATGACAGCTGCAACAACGATACCTTCTCCAAATAAGCGAGAAGCGCTAAATATCAGAATTAAACCGGAAGAACGGGATTT
AATAGATCGAGCAGCAAAGATTCAGAGAAAAAGTCGTACTAATTTTGTATTGGAAGCTGCCTGTAGAGCCGCAGAAGAAG
CGCTTTTCGATCAATGTCTCATCTCTGTTGATCCAAAGGCGTATACTGATTTTTTAATGCGCCTCGATATGCCGCCACGC
CCTAATGATCGGCTGCAAAAAACCATGCAAACGGTAGCGCCGTGGGATGAGCTGTGA