Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 59216..59859 Replicon plasmid unnamed2
Accession NZ_CP036447
Organism Klebsiella pneumoniae strain KPNIH45

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag C3377_RS00930 Protein ID WP_000754566.1
Coordinates 59216..59632 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag C3377_RS00935 Protein ID WP_001261276.1
Coordinates 59629..59859 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3377_RS00905 54773..55632 - 860 Protein_69 IS3 family transposase -
C3377_RS00910 55716..56051 + 336 WP_011977766.1 C2H2-type zinc finger protein -
C3377_RS00915 56798..57874 - 1077 WP_000227969.1 IS110 family transposase -
C3377_RS00925 58156..59012 - 857 Protein_72 IS3-like element ISEc15 family transposase -
C3377_RS00930 59216..59632 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
C3377_RS00935 59629..59859 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C3377_RS00945 60433..60783 + 351 WP_000493378.1 hypothetical protein -
C3377_RS00950 60834..61577 + 744 WP_000129823.1 hypothetical protein -
C3377_RS00955 61574..62350 + 777 WP_000015958.1 site-specific integrase -
C3377_RS00960 62408..62665 - 258 WP_000764642.1 hypothetical protein -
C3377_RS28630 62794..62898 - 105 WP_032409716.1 hypothetical protein -
C3377_RS00965 63428..64294 + 867 WP_004118283.1 replication initiation protein -
C3377_RS00970 64471..64740 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid sul3 / dfrA12 / aadA2 / qacE / sul1 / mph(A) / cmlA1 / ant(3'')-Ia - 1..87465 87465
- inside Integron - - 5..87465 87460


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T119821 WP_000754566.1 NZ_CP036447:c59632-59216 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T119821 NZ_CP036447:c59632-59216 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT119821 WP_001261276.1 NZ_CP036447:c59859-59629 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT119821 NZ_CP036447:c59859-59629 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References