Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 189096..189721 Replicon chromosome
Accession NZ_CP036442
Organism Klebsiella pneumoniae strain ABFPV

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag WN05_RS00950 Protein ID WP_002882817.1
Coordinates 189338..189721 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag WN05_RS00945 Protein ID WP_004150355.1
Coordinates 189096..189338 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WN05_RS00920 185087..185686 + 600 WP_004151865.1 glucose-1-phosphatase -
WN05_RS00925 185680..186540 + 861 WP_004146232.1 virulence factor BrkB family protein -
WN05_RS00930 186537..186974 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
WN05_RS00935 187019..187960 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
WN05_RS00940 187974..188891 - 918 WP_009484979.1 alpha/beta hydrolase -
WN05_RS00945 189096..189338 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
WN05_RS00950 189338..189721 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
WN05_RS00955 189895..190824 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
WN05_RS00960 190821..191456 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
WN05_RS00965 191453..192355 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T119803 WP_002882817.1 NZ_CP036442:189338-189721 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T119803 NZ_CP036442:189338-189721 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT119803 WP_004150355.1 NZ_CP036442:189096-189338 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT119803 NZ_CP036442:189096-189338 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References