Detailed information of TA system TA119801

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 61605..62341
• Replicon: plasmid unnamed1
• Accession: NZ_CP036439
• Organism: Klebsiella pneumoniae strain ABFQB

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: WN11_RS26350
• Protein ID: WP_131138837.1
• Coordinates: 61605..62087 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: WN11_RS26355
• Protein ID: WP_003026799.1
• Coordinates: 62075..62341 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WN11_RS26325	57010..58248	-	1239	WP_131138834.1	bifunctional succinylornithine transaminase/acetylornithine transaminase	-
WN11_RS26330	58628..59545	-	918	WP_131138835.1	amidinotransferase	-
WN11_RS26335	59568..60581	-	1014	WP_131138836.1	ornithine cyclodeaminase	-
WN11_RS26340	60706..61128	+	423	WP_049095386.1	Lrp/AsnC family transcriptional regulator	-
WN11_RS26345	61150..61416	+	267	Protein_60	transposase	-
WN11_RS26350	61605..62087	-	483	WP_131138837.1	GNAT family N-acetyltransferase	Toxin
WN11_RS26355	62075..62341	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
WN11_RS26360	62602..63492	+	891	WP_131138838.1	hypothetical protein	-
WN11_RS26365	63558..63668	-	111	Protein_64	hypothetical protein	-
WN11_RS26370	63715..63981	-	267	WP_131138839.1	hypothetical protein	-
WN11_RS26380	64757..65248	-	492	WP_131138840.1	DNA-binding protein	-
WN11_RS26385	65282..65848	-	567	WP_131138876.1	hypothetical protein	-
WN11_RS26390	65845..66105	-	261	WP_131138841.1	hypothetical protein	-
WN11_RS26395	66735..66980	-	246	WP_131138842.1	hypothetical protein	-
WN11_RS26400	67020..67292	-	273	WP_131138843.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..115291	115291

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (58-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17267.98 Da
• Isoelectric Point: 9.5893

>T119801 WP_131138837.1 NZ_CP036439:c62087-61605 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFIVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEKAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T119801 NZ_CP036439:c62087-61605 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGGGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACGTTCATTGTTTGCAAGACGGGTACAA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGTGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAAAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAGCTACCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT119801 WP_003026799.1 NZ_CP036439:c62341-62075 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT119801 NZ_CP036439:c62341-62075 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTGAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCTGCGGCAGAAATCCTTCA
TAAATCGCGAACTGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66