Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 260114..260745 Replicon chromosome
Accession NZ_CP036438
Organism Klebsiella pneumoniae strain ABFQB

Toxin (Protein)


Gene name hicA Uniprot ID A0A1Q8YTV3
Locus tag WN11_RS01395 Protein ID WP_012542177.1
Coordinates 260569..260745 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A2X1QFN9
Locus tag WN11_RS01390 Protein ID WP_012542176.1
Coordinates 260114..260521 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WN11_RS01360 256346..256891 - 546 WP_119127038.1 terminase small subunit -
WN11_RS01365 257185..257499 - 315 WP_119127047.1 hypothetical protein -
WN11_RS01370 257604..257912 - 309 WP_131138699.1 hypothetical protein -
WN11_RS01375 258902..259252 - 351 WP_119127040.1 hypothetical protein -
WN11_RS01380 259249..259746 - 498 WP_119127041.1 lysozyme -
WN11_RS01385 259746..259961 - 216 WP_016160648.1 class II holin family protein -
WN11_RS01390 260114..260521 - 408 WP_012542176.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
WN11_RS01395 260569..260745 - 177 WP_012542177.1 type II toxin-antitoxin system HicA family toxin Toxin
WN11_RS01400 261115..261717 - 603 WP_119127043.1 hypothetical protein -
WN11_RS01405 261734..262765 - 1032 WP_119127044.1 DUF968 domain-containing protein -
WN11_RS01415 262965..263357 - 393 WP_119127045.1 DNA-binding protein -
WN11_RS01420 263398..263637 - 240 WP_071785642.1 hypothetical protein -
WN11_RS01425 263700..263933 - 234 WP_046622338.1 DinI-like family protein -
WN11_RS01435 265435..265638 - 204 WP_046622340.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 224552..282765 58213


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

HicB-like antitoxin of toxin-antitoxin system

(3-61)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6632.83 Da        Isoelectric Point: 11.0174

>T119788 WP_012542177.1 NZ_CP036438:c260745-260569 [Klebsiella pneumoniae]
VKQSELRRWLAAQGAEFKDGTNHLKIYLNGKQTVMPRHPGKEIPEPLRKAILKQLGIK

Download         Length: 177 bp

>T119788 NZ_CP036438:c260745-260569 [Klebsiella pneumoniae]
GTGAAGCAAAGCGAGTTGAGGCGTTGGCTTGCGGCTCAAGGGGCAGAATTTAAAGATGGTACTAACCACTTGAAAATCTA
TCTCAACGGCAAGCAAACGGTAATGCCGAGACATCCGGGGAAGGAAATACCGGAACCGCTGAGGAAGGCAATTCTTAAGC
AACTCGGCATCAAATAA

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 14977.99 Da        Isoelectric Point: 4.4277

>AT119788 WP_012542176.1 NZ_CP036438:c260521-260114 [Klebsiella pneumoniae]
MRYPVIFEHDETGWAVFFPDIPEAMTGGETREEALEMAQDALVTAFDFYFDDRREIPAPSAEGDAFVEVPASVAAKVLLL
NRLVSTNTSNADLARMINTRPQEVQRIVSLGHSTKIDTIQKALSALGQKMEIVVH

Download         Length: 408 bp

>AT119788 NZ_CP036438:c260521-260114 [Klebsiella pneumoniae]
ATGCGATACCCGGTAATTTTTGAGCATGACGAAACCGGCTGGGCGGTATTCTTTCCGGATATCCCAGAGGCAATGACGGG
AGGAGAGACCAGGGAGGAGGCGTTAGAAATGGCGCAAGATGCCCTGGTGACGGCGTTTGATTTTTACTTTGACGACCGCC
GGGAGATTCCCGCTCCATCAGCAGAAGGTGATGCGTTTGTTGAGGTTCCGGCCAGCGTGGCAGCTAAAGTATTGCTGTTA
AACCGTCTGGTCAGCACCAACACCAGCAATGCTGATCTCGCCCGCATGATTAACACGCGCCCACAGGAAGTGCAGCGCAT
CGTGTCTCTTGGTCACAGCACCAAAATCGATACGATCCAAAAAGCGCTATCAGCGTTGGGGCAAAAGATGGAAATTGTCG
TCCACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q8YTV3

Antitoxin

Source ID Structure
AlphaFold DB A0A2X1QFN9

References