Detailed information of TA system TA119693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 660872..661304
• Replicon: chromosome
• Accession: NZ_CP036376
• Organism: Streptococcus agalactiae strain Sag153

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: EXA24_RS03605
• Protein ID: WP_001284310.1
• Coordinates: 661146..661304 (+)
• Length: 53 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: EXA24_RS03600
• Protein ID: WP_000301765.1
• Coordinates: 660872..661144 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA24_RS03560	656528..656779	+	252	Protein_620	GNAT family N-acetyltransferase	-
EXA24_RS03565	656872..657666	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
EXA24_RS03570	658076..658159	+	84	Protein_622	peptide-binding protein	-
EXA24_RS03575	658208..658291	+	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
EXA24_RS03580	658416..659153	+	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
EXA24_RS03585	659299..659583	+	285	WP_073459356.1	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
EXA24_RS03590	659651..660547	+	897	WP_001809760.1	ParA family protein	-
EXA24_RS03595	660645..660854	+	210	WP_000527318.1	peptide-binding protein	-
EXA24_RS03600	660872..661144	+	273	WP_000301765.1	antitoxin	Antitoxin
EXA24_RS03605	661146..661304	+	159	WP_001284310.1	zeta toxin family protein	Toxin
EXA24_RS03610	661356..661901	+	546	WP_001071501.1	AAA family ATPase	-
EXA24_RS03615	662077..662943	+	867	WP_001255868.1	aminoglycoside 6-adenylyltransferase AadE	-
EXA24_RS03630	663514..664182	+	669	Protein_632	zeta toxin family protein	-
EXA24_RS03635	664333..664815	+	483	Protein_633	hypothetical protein	-
EXA24_RS03640	664816..665981	+	1166	Protein_634	type IV secretory system conjugative DNA transfer family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / ant(6)-Ia / aph(3')-III / erm(B)	gbs0628 / gbs0630	591400..697611	106211
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	654022..662943	8921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (26-50)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 5790.55 Da
• Isoelectric Point: 10.3088

>T119693 WP_001284310.1 NZ_CP036376:661146-661304 [Streptococcus agalactiae]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRRR

Nucleotide

• Length: 159 bp

>T119693 NZ_CP036376:661146-661304 [Streptococcus agalactiae]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGAAGAAGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT119693 WP_000301765.1 NZ_CP036376:660872-661144 [Streptococcus agalactiae]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT119693 NZ_CP036376:660872-661144 [Streptococcus agalactiae]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3