Detailed information of TA system TA119692

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 652166..653274
• Replicon: chromosome
• Accession: NZ_CP036376
• Organism: Streptococcus agalactiae strain Sag153

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: EXA24_RS03535
• Protein ID: WP_071661597.1
• Coordinates: 652405..653274 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: EXA24_RS03530
• Protein ID: WP_000205227.1
• Coordinates: 652166..652390 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXA24_RS03495	647379..647552	+	174	WP_002779755.1	cysteine-rich KTR domain-containing protein	-
EXA24_RS03500	647755..647925	+	171	WP_000713595.1	hypothetical protein	-
EXA24_RS03505	647939..648541	+	603	WP_001062586.1	recombinase family protein	-
EXA24_RS03510	648557..649234	+	678	Protein_609	DNA topoisomerase	-
EXA24_RS11145	649545..649709	+	165	Protein_610	DNA recombinase	-
EXA24_RS03515	649746..650042	+	297	WP_002360685.1	hypothetical protein	-
EXA24_RS03520	650043..650459	+	417	WP_000323438.1	recombinase	-
EXA24_RS03525	650479..652023	+	1545	WP_002390960.1	recombinase family protein	-
EXA24_RS03530	652166..652390	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
EXA24_RS03535	652405..653274	+	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
EXA24_RS03540	653255..653989	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
EXA24_RS03545	654022..654930	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
EXA24_RS03550	654927..655133	+	207	WP_044116541.1	hypothetical protein	-
EXA24_RS03555	655207..656369	+	1163	WP_088186165.1	IS3-like element ISSag12 family transposase	-
EXA24_RS03560	656528..656779	+	252	Protein_620	GNAT family N-acetyltransferase	-
EXA24_RS03565	656872..657666	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
EXA24_RS03570	658076..658159	+	84	Protein_622	peptide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / ant(6)-Ia / aph(3')-III / erm(B)	gbs0628 / gbs0630	591400..697611	106211
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	654022..662943	8921

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T119692 WP_071661597.1 NZ_CP036376:652405-653274 [Streptococcus agalactiae]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T119692 NZ_CP036376:652405-653274 [Streptococcus agalactiae]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGTATCAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAACATAAACCTGAAAAATATACCGAG
AAGGTAAATCATATTTTTGAAGTACTCGGTATCTCACTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT119692 WP_000205227.1 NZ_CP036376:652166-652390 [Streptococcus agalactiae]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT119692 NZ_CP036376:652166-652390 [Streptococcus agalactiae]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6