Detailed information of TA system TA119689

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1958..2432
• Replicon: plasmid p3_020037
• Accession: NZ_CP036375
• Organism: Klebsiella pneumoniae strain WCHKP020037

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: A0A8J3DT22
• Locus tag: CEA76_RS29610
• Protein ID: WP_004197762.1
• Coordinates: 1958..2224 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: S1EJF5
• Locus tag: CEA76_RS29615
• Protein ID: WP_000879771.1
• Coordinates: 2208..2432 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CEA76_RS29595	522..901	+	380	Protein_0	hypothetical protein	-
CEA76_RS29600	927..1472	+	546	WP_023317533.1	hypothetical protein	-
CEA76_RS29605	1538..1888	+	351	WP_004197756.1	hypothetical protein	-
CEA76_RS29610	1958..2224	-	267	WP_004197762.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CEA76_RS29615	2208..2432	-	225	WP_000879771.1	TraY domain-containing protein	Antitoxin
CEA76_RS29620	3099..3311	-	213	WP_021527181.1	MbeD family mobilization/exclusion protein	-
CEA76_RS29625	3318..3827	-	510	WP_032433972.1	MbeB family mobilization protein	-
CEA76_RS29630	3802..4566	-	765	Protein_7	relaxase/mobilization nuclease domain-containing protein	-
CEA76_RS29635	4505..4849	-	345	WP_062955149.1	MobC family plasmid mobilization relaxosome protein	-
CEA76_RS30975	4839..5015	-	177	WP_004197757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..5596	5596

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10342.96 Da
• Isoelectric Point: 9.8727

>T119689 WP_004197762.1 NZ_CP036375:c2224-1958 [Klebsiella pneumoniae]
MVWTINYSDRALKSLRKMDKQNARRIVDFMSLRIAVAADPRQSGKPLKGELGEFWRYRVGDYRVLCEIRDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T119689 NZ_CP036375:c2224-1958 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.28 Da
• Isoelectric Point: 4.6533

>AT119689 WP_000879771.1 NZ_CP036375:c2432-2208 [Klebsiella pneumoniae]
MLAIRLSDEIESRLDSLAKQTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT119689 NZ_CP036375:c2432-2208 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: S1EJF5