Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 94644..95287 Replicon plasmid pKPC2_020037
Accession NZ_CP036372
Organism Klebsiella pneumoniae strain WCHKP020037

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CEA76_RS28290 Protein ID WP_001044770.1
Coordinates 94644..95060 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CEA76_RS28295 Protein ID WP_001261282.1
Coordinates 95057..95287 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEA76_RS28270 90747..91019 - 273 Protein_110 transposase -
CEA76_RS28275 91048..91745 - 698 Protein_111 IS1 family transposase -
CEA76_RS28280 92001..93023 - 1023 WP_000361404.1 helicase UvrD -
CEA76_RS28285 93008..94570 - 1563 WP_004206609.1 AAA family ATPase -
CEA76_RS28290 94644..95060 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CEA76_RS28295 95057..95287 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CEA76_RS28300 95244..95705 + 462 WP_014343465.1 hypothetical protein -
CEA76_RS28305 95866..96810 + 945 WP_011977810.1 hypothetical protein -
CEA76_RS28310 96847..97239 + 393 WP_011977811.1 hypothetical protein -
CEA76_RS28315 97297..97818 + 522 WP_013214008.1 hypothetical protein -
CEA76_RS28320 97864..98067 + 204 WP_011977813.1 hypothetical protein -
CEA76_RS28325 98097..99101 + 1005 WP_011977814.1 hypothetical protein -
CEA76_RS28330 99285..100064 + 780 WP_013214009.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-65 / fosA3 / blaTEM-1B / rmtB / blaKPC-2 / catA2 - 1..172770 172770


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T119685 WP_001044770.1 NZ_CP036372:c95060-94644 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T119685 NZ_CP036372:c95060-94644 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT119685 WP_001261282.1 NZ_CP036372:c95287-95057 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT119685 NZ_CP036372:c95287-95057 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References