Detailed information of TA system TA119661

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4814..5394
• Replicon: plasmid p2_115068
• Accession: NZ_CP036369
• Organism: Klebsiella pneumoniae strain WCHKP115068

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: DVJ72_RS28780
• Protein ID: WP_071177730.1
• Coordinates: 5080..5394 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: DVJ72_RS28775
• Protein ID: WP_000093040.1
• Coordinates: 4814..5092 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DVJ72_RS28745	2..1150	-	1149	Protein_0	cloacin	-
DVJ72_RS28750	1255..2742	-	1488	WP_004178082.1	group II intron reverse transcriptase/maturase	-
DVJ72_RS28760	3387..3632	+	246	WP_032440458.1	hypothetical protein	-
DVJ72_RS28765	3906..4277	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
DVJ72_RS28770	4274..4480	+	207	Protein_4	TonB family protein	-
DVJ72_RS28775	4814..5092	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
DVJ72_RS28780	5080..5394	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DVJ72_RS28785	5558..5986	+	429	WP_001140599.1	hypothetical protein	-
DVJ72_RS28790	6012..6191	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
DVJ72_RS28795	6218..6748	-	531	WP_071177729.1	hypothetical protein	-
DVJ72_RS28800	6755..7486	-	732	WP_071177728.1	replication-relaxation family protein	-
DVJ72_RS28805	7486..9450	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..12746	12746

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T119661 WP_071177730.1 NZ_CP036369:5080-5394 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T119661 NZ_CP036369:5080-5394 [Klebsiella pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT119661 WP_000093040.1 NZ_CP036369:4814-5092 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT119661 NZ_CP036369:4814-5092 [Klebsiella pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1