Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4165830..4166449 Replicon chromosome
Accession NZ_CP036361
Organism Klebsiella pneumoniae strain WCHKP2080

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag B7D45_RS21065 Protein ID WP_002892050.1
Coordinates 4166231..4166449 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag B7D45_RS21060 Protein ID WP_002892066.1
Coordinates 4165830..4166204 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7D45_RS21050 4160982..4162175 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
B7D45_RS21055 4162198..4165344 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit -
B7D45_RS21060 4165830..4166204 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
B7D45_RS21065 4166231..4166449 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
B7D45_RS21070 4166608..4167174 + 567 WP_002892030.1 maltose O-acetyltransferase -
B7D45_RS29590 4167146..4167286 - 141 WP_004147370.1 hypothetical protein -
B7D45_RS21075 4167307..4167777 + 471 WP_002892026.1 YlaC family protein -
B7D45_RS21080 4167752..4169203 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
B7D45_RS21085 4169304..4170002 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
B7D45_RS21090 4169999..4170139 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
B7D45_RS21095 4170139..4170402 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T119630 WP_002892050.1 NZ_CP036361:4166231-4166449 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T119630 NZ_CP036361:4166231-4166449 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT119630 WP_002892066.1 NZ_CP036361:4165830-4166204 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT119630 NZ_CP036361:4165830-4166204 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References