Detailed information of TA system TA119616

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 368886..369556
• Replicon: plasmid p_JBx_073812
• Accession: NZ_CP036360
• Organism: Agrobacterium sp. 33MFTa1.1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EYD00_RS25160
• Protein ID: WP_006699040.1
• Coordinates: 368886..369305 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U4Q725
• Locus tag: EYD00_RS25165
• Protein ID: WP_006699039.1
• Coordinates: 369302..369556 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYD00_RS25135	363994..365184	+	1191	WP_034499698.1	hypothetical protein	-
EYD00_RS25140	365291..365545	+	255	WP_034499700.1	hypothetical protein	-
EYD00_RS25145	365673..366125	+	453	WP_131258966.1	hypothetical protein	-
EYD00_RS25150	366138..367175	+	1038	WP_034499702.1	toprim domain-containing protein	-
EYD00_RS25155	367505..368446	+	942	WP_034499705.1	DUF2493 domain-containing protein	-
EYD00_RS25160	368886..369305	-	420	WP_006699040.1	type II toxin-antitoxin system VapC family toxin	Toxin
EYD00_RS25165	369302..369556	-	255	WP_006699039.1	hypothetical protein	Antitoxin
EYD00_RS25170	369725..369955	+	231	WP_128636328.1	hypothetical protein	-
EYD00_RS25175	370007..370513	-	507	WP_034499708.1	RES domain-containing protein	-
EYD00_RS25180	370510..370995	-	486	WP_034499710.1	DUF2384 domain-containing protein	-
EYD00_RS25185	371401..371724	+	324	WP_034499714.1	DUF736 family protein	-
EYD00_RS25190	371781..372113	+	333	WP_034499718.1	hypothetical protein	-
EYD00_RS25195	372179..372457	-	279	WP_006699033.1	HU family DNA-binding protein	-
EYD00_RS26045	372856..373020	+	165	WP_022557193.1	hypothetical protein	-
EYD00_RS25200	373234..373485	-	252	Protein_373	WGR domain-containing protein	-
EYD00_RS25205	373596..374231	-	636	Protein_374	extracellular solute-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..496948	496948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-123)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15050.15 Da
• Isoelectric Point: 4.8728

>T119616 WP_006699040.1 NZ_CP036360:c369305-368886 [Agrobacterium sp. 33MFTa1.1]
MILLDTNVVSETMKPFPSQMVQRWLDEQAAETLHLSSITIAEAMFGIRTMPDGQRKQRLAEALDVWIGLFEGRILPFDLD
AAKHYANLGSSARASGKGFPTPDGYIAAIAASKGFAVATRDASAFHAAGLEVIDPWTAE

Nucleotide

• Length: 420 bp

>T119616 NZ_CP036360:c369305-368886 [Agrobacterium sp. 33MFTa1.1]
ATGATTCTGCTAGACACCAATGTCGTCTCGGAGACGATGAAGCCTTTTCCGAGCCAAATGGTACAGCGCTGGCTCGACGA
GCAAGCTGCCGAGACCCTCCATCTTTCGAGTATCACGATCGCGGAGGCCATGTTCGGCATCCGCACCATGCCCGATGGTC
AGCGCAAGCAGAGGCTCGCGGAGGCTCTCGATGTCTGGATCGGCCTGTTCGAGGGACGAATTTTGCCCTTCGATCTCGAT
GCCGCCAAACACTATGCCAATCTCGGATCTTCGGCCCGTGCATCGGGGAAGGGATTCCCGACACCCGACGGCTACATTGC
GGCCATCGCTGCATCTAAGGGCTTCGCAGTCGCGACCCGTGACGCCAGCGCGTTTCATGCCGCCGGTCTTGAAGTCATCG
ATCCCTGGACGGCCGAGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9406.71 Da
• Isoelectric Point: 6.7270

>AT119616 WP_006699039.1 NZ_CP036360:c369556-369302 [Agrobacterium sp. 33MFTa1.1]
MPAVTIRNIPEETHRAIKIRAAQHGRSAEAEMRDILEMAVRPATRLRLGTALAEASRRIGLTNEDVDALEQTFDRTPAHP
MSFE

Nucleotide

• Length: 255 bp

>AT119616 NZ_CP036360:c369556-369302 [Agrobacterium sp. 33MFTa1.1]
ATGCCCGCCGTAACAATCCGAAACATACCCGAGGAAACGCATCGCGCCATCAAGATCCGTGCGGCACAGCATGGCCGCAG
CGCCGAGGCCGAAATGCGCGACATTCTGGAAATGGCCGTTCGGCCTGCAACCCGTCTCCGTCTCGGCACGGCACTCGCCG
AGGCCAGTCGTCGCATCGGCCTCACCAACGAGGACGTCGATGCCCTTGAGCAGACCTTTGATCGCACGCCAGCCCATCCC
ATGAGCTTCGAATGA

Structures

Antitoxin

• AlphaFold DB: U4Q725