Detailed information of TA system TA119615

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 192161..192759
• Replicon: plasmid p_JBx_073812
• Accession: NZ_CP036360
• Organism: Agrobacterium sp. 33MFTa1.1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: U4QIX0
• Locus tag: EYD00_RS24250
• Protein ID: WP_022557667.1
• Coordinates: 192466..192759 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U4Q8S4
• Locus tag: EYD00_RS24245
• Protein ID: WP_022557668.1
• Coordinates: 192161..192469 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYD00_RS24220	187330..188043	-	714	WP_022557673.1	IS6 family transposase	-
EYD00_RS24225	188128..189159	+	1032	WP_081859572.1	polyamine ABC transporter substrate-binding protein	-
EYD00_RS24230	189532..189918	-	387	Protein_182	IS6 family transposase	-
EYD00_RS24235	190153..190743	+	591	WP_022557670.1	helix-turn-helix transcriptional regulator	-
EYD00_RS24240	190736..191965	+	1230	WP_034499453.1	HipA domain-containing protein	-
EYD00_RS24245	192161..192469	+	309	WP_022557668.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
EYD00_RS24250	192466..192759	+	294	WP_022557667.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
EYD00_RS24255	193279..195213	+	1935	WP_034499455.1	HAMP domain-containing protein	-
EYD00_RS24260	195467..195766	-	300	WP_034499457.1	hypothetical protein	-
EYD00_RS24265	196215..196466	+	252	WP_034499459.1	antitoxin	-
EYD00_RS24270	196463..196774	+	312	WP_022557664.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EYD00_RS24280	197284..197484	-	201	WP_006699143.1	type II toxin-antitoxin system VapB family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	htpB	1..496948	496948

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (4-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10505.03 Da
• Isoelectric Point: 5.7996

>T119615 WP_022557667.1 NZ_CP036360:192466-192759 [Agrobacterium sp. 33MFTa1.1]
VKLTWSAFALSDRDAIFTYVEADNPAAAVLIDERIAAAARRLLDFPASGRVGRIAGTRELVINGTPYIAAYAVAETAVRI
LRVLHGAQEWPENLPLS

Nucleotide

• Length: 294 bp

>T119615 NZ_CP036360:192466-192759 [Agrobacterium sp. 33MFTa1.1]
GTGAAGCTTACTTGGTCTGCTTTTGCGTTGTCGGATCGTGACGCCATCTTCACATACGTCGAGGCAGATAATCCCGCCGC
TGCCGTGTTGATCGATGAACGGATAGCCGCTGCCGCCCGTCGCTTGCTCGATTTTCCTGCCAGTGGGCGCGTGGGTCGAA
TTGCCGGGACACGCGAACTCGTCATCAATGGCACGCCTTATATCGCCGCCTACGCCGTTGCCGAGACGGCAGTTCGAATT
CTTCGCGTACTCCATGGCGCGCAAGAATGGCCAGAAAATCTGCCCCTAAGCTGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11098.46 Da
• Isoelectric Point: 5.1704

>AT119615 WP_022557668.1 NZ_CP036360:192161-192469 [Agrobacterium sp. 33MFTa1.1]
MAANALVQTRIDSEVRDRASAVLESMGLTVSDAVRIMLTRTANEGALPLELLSGSEAHDAWFRTKVHEALNDTRPDLSDD
EAEAHFVKRRAAARLKAGVPES

Nucleotide

• Length: 309 bp

>AT119615 NZ_CP036360:192161-192469 [Agrobacterium sp. 33MFTa1.1]
ATGGCCGCAAACGCTCTCGTACAAACACGTATTGATTCCGAAGTCAGGGATCGTGCTTCTGCCGTCCTTGAAAGCATGGG
CCTCACGGTATCTGATGCGGTTCGTATCATGCTCACCCGGACCGCTAACGAGGGCGCTCTGCCTCTTGAACTTTTATCCG
GCAGTGAGGCGCATGACGCTTGGTTTCGGACCAAGGTGCATGAAGCGTTAAACGATACTCGACCGGATTTATCGGACGAC
GAGGCCGAGGCGCACTTTGTCAAACGTCGAGCGGCAGCCCGCCTTAAAGCGGGTGTGCCAGAATCGTGA

Structures

Toxin

• AlphaFold DB: U4QIX0

Antitoxin

• AlphaFold DB: U4Q8S4