Detailed information of TA system TA119614

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 2039399..2040005
• Replicon: chromosome
• Accession: NZ_CP036358
• Organism: Agrobacterium sp. 33MFTa1.1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A7W4TY99
• Locus tag: EYD00_RS10365
• Protein ID: WP_004443439.1
• Coordinates: 2039399..2039695 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: EYD00_RS10370
• Protein ID: WP_004443440.1
• Coordinates: 2039700..2040005 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYD00_RS10330	2035188..2036081	-	894	WP_004443429.1	hemin ABC transporter substrate-binding protein	-
EYD00_RS10335	2036268..2036615	+	348	WP_004429958.1	antibiotic biosynthesis monooxygenase	-
EYD00_RS10340	2036625..2037149	+	525	WP_004443430.1	heme utilization cystosolic carrier protein HutX	-
EYD00_RS10350	2037485..2038261	+	777	WP_004443433.1	YbaY family lipoprotein	-
EYD00_RS10360	2038469..2039353	+	885	WP_006700424.1	formyltetrahydrofolate deformylase	-
EYD00_RS10365	2039399..2039695	+	297	WP_004443439.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EYD00_RS10370	2039700..2040005	+	306	WP_004443440.1	putative addiction module antidote protein	Antitoxin
EYD00_RS10375	2040017..2041411	-	1395	WP_004443442.1	sensor histidine kinase	-
EYD00_RS10380	2041408..2042088	-	681	WP_004443444.1	response regulator transcription factor	-
EYD00_RS10385	2042158..2043237	+	1080	WP_077987473.1	ABC transporter substrate-binding protein	-
EYD00_RS10390	2043588..2044532	+	945	WP_004443448.1	tripartite tricarboxylate transporter substrate binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-90)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10923.61 Da
• Isoelectric Point: 10.2206

>T119614 WP_004443439.1 NZ_CP036358:2039399-2039695 [Agrobacterium sp. 33MFTa1.1]
MLTIRETSEFADWLAGLRDLQARARIARRIYRLADGNPGDVKPVGEGVSELRIDFGPGYRVYFFRNGDVVIILLCGGDKA
SQSRDIAKAKKLARALKE

Nucleotide

• Length: 297 bp

>T119614 NZ_CP036358:2039399-2039695 [Agrobacterium sp. 33MFTa1.1]
ATGCTCACGATCCGGGAAACAAGCGAGTTTGCCGATTGGCTTGCCGGTCTGCGCGATCTGCAGGCGCGTGCCCGCATCGC
ACGGCGCATTTACAGGCTGGCAGACGGCAATCCAGGAGACGTAAAGCCGGTCGGCGAAGGCGTCAGTGAGCTCAGGATCG
ATTTTGGACCCGGATATCGGGTCTATTTCTTCCGGAACGGCGATGTCGTGATCATATTGCTGTGCGGTGGCGACAAGGCA
TCACAGTCCCGCGATATTGCCAAAGCCAAGAAATTGGCCCGAGCACTCAAGGAATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10547.84 Da
• Isoelectric Point: 4.5580

>AT119614 WP_004443440.1 NZ_CP036358:2039700-2040005 [Agrobacterium sp. 33MFTa1.1]
MPLVTRDYDAARYLDSDEALAAYIADATENGDAQELAHALGVVARAKGITDLSRQTGLPRQTLYKALSGEGNPELATIAK
VADALGYKLSLVAKSPADTAA

Nucleotide

• Length: 306 bp

>AT119614 NZ_CP036358:2039700-2040005 [Agrobacterium sp. 33MFTa1.1]
ATGCCACTTGTGACACGCGATTACGATGCTGCCCGCTATCTTGATAGTGACGAAGCACTTGCCGCCTACATAGCAGACGC
CACTGAGAACGGCGACGCACAGGAGCTTGCCCACGCTCTCGGCGTCGTGGCGCGAGCCAAAGGCATAACCGATCTTTCGA
GACAGACCGGCCTTCCCCGTCAGACGTTGTACAAGGCGCTCAGCGGCGAGGGAAACCCCGAACTGGCAACGATCGCCAAG
GTCGCCGATGCATTGGGCTACAAACTTTCCCTGGTGGCGAAATCACCTGCGGATACAGCCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A7W4TY99