Detailed information of TA system TA119613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 495905..496499
• Replicon: chromosome
• Accession: NZ_CP036358
• Organism: Agrobacterium sp. 33MFTa1.1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: EYD00_RS02465
• Protein ID: WP_004440489.1
• Coordinates: 496185..496499 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EYD00_RS02460
• Protein ID: WP_004440487.1
• Coordinates: 495905..496204 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYD00_RS02435	491040..492038	+	999	WP_004440476.1	asparaginase	-
EYD00_RS02440	492173..492793	+	621	WP_004440479.1	glutathione S-transferase family protein	-
EYD00_RS02445	492947..493606	-	660	WP_004440482.1	glutathione S-transferase family protein	-
EYD00_RS02450	493689..494204	-	516	WP_004440484.1	MarR family transcriptional regulator	-
EYD00_RS02455	494346..495875	+	1530	WP_004440486.1	MFS transporter	-
EYD00_RS02460	495905..496204	-	300	WP_004440487.1	putative addiction module antidote protein	Antitoxin
EYD00_RS02465	496185..496499	-	315	WP_004440489.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EYD00_RS02470	496739..497749	+	1011	WP_034496274.1	ligase-associated DNA damage response exonuclease	-
EYD00_RS02475	497751..499376	+	1626	WP_004440496.1	cisplatin damage response ATP-dependent DNA ligase	-
EYD00_RS02480	499548..500513	+	966	WP_034495910.1	S9 family peptidase	-
EYD00_RS02485	500549..501333	-	785	Protein_474	mannosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-95)

Antitoxin

• Uncharacterized protein HI1420 (3-97)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11746.36 Da
• Isoelectric Point: 9.5802

>T119613 WP_004440489.1 NZ_CP036358:c496499-496185 [Agrobacterium sp. 33MFTa1.1]
MNDFEKSAEFDEWLKALKDRTGKARILHRLTAAEHGNFGDCEPVGGGVSEMRIHCGPGYRVYFTRIGKRVYFLLVGGDKS
SQKRDIKRAVEMAQTIGRNDRDAV

Nucleotide

• Length: 315 bp

>T119613 NZ_CP036358:c496499-496185 [Agrobacterium sp. 33MFTa1.1]
ATGAACGATTTCGAGAAATCGGCAGAGTTCGACGAATGGCTGAAGGCTTTGAAAGATCGCACCGGAAAGGCGCGAATTCT
TCATCGCCTTACCGCGGCCGAGCACGGGAATTTCGGCGACTGCGAGCCGGTCGGTGGGGGTGTTTCAGAAATGCGCATCC
ACTGTGGTCCGGGTTATCGGGTCTATTTCACACGCATTGGCAAGAGGGTGTATTTCCTTCTGGTCGGCGGAGACAAATCC
TCGCAGAAGCGGGACATAAAGCGGGCAGTCGAGATGGCGCAAACAATAGGAAGGAATGACCGTGACGCAGTTTAG

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10755.25 Da
• Isoelectric Point: 5.6863

>AT119613 WP_004440487.1 NZ_CP036358:c496204-495905 [Agrobacterium sp. 33MFTa1.1]
MTVTQFSKFDVSDYLNSDEAMLEYLAVAMEDENPDVFIAALGAVARARGMSHVADAAGLGRESLYKALKPGSKLRYETVQ
KILHSLGFRLAVEKSSNAA

Nucleotide

• Length: 300 bp

>AT119613 NZ_CP036358:c496204-495905 [Agrobacterium sp. 33MFTa1.1]
ATGACCGTGACGCAGTTTAGCAAATTCGATGTGTCGGACTACCTGAATAGCGATGAAGCCATGCTCGAATACCTGGCAGT
GGCGATGGAAGACGAGAACCCCGACGTCTTCATCGCCGCCCTTGGCGCGGTCGCCAGAGCTCGCGGAATGTCGCATGTCG
CGGATGCCGCAGGTCTCGGCCGGGAAAGTCTCTATAAGGCACTTAAGCCCGGTTCGAAGCTCCGCTACGAGACCGTGCAG
AAAATCCTGCATTCGCTAGGCTTCCGACTGGCCGTCGAAAAGTCATCGAACGCCGCGTAA