Detailed information of TA system TA119606

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 98161..98804
• Replicon: plasmid pIncFIBK
• Accession: NZ_CP036337
• Organism: Klebsiella pneumoniae strain BP327

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: EYB55_RS28790
• Protein ID: WP_014386165.1
• Coordinates: 98388..98804 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: EYB55_RS28785
• Protein ID: WP_032408893.1
• Coordinates: 98161..98391 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYB55_RS28755	93235..94230	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
EYB55_RS28760	94237..95385	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
EYB55_RS28765	95556..96713	-	1158	WP_032408895.1	acetylornithine deacetylase	-
EYB55_RS28770	96729..97402	-	674	Protein_107	DUF1028 domain-containing protein	-
EYB55_RS28775	97407..97841	-	435	WP_000103648.1	RidA family protein	-
EYB55_RS28785	98161..98391	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
EYB55_RS28790	98388..98804	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
EYB55_RS28795	99127..100095	+	969	WP_074422984.1	IS5 family transposase	-
EYB55_RS28800	100275..100649	-	375	WP_032408891.1	hypothetical protein	-
EYB55_RS28805	100705..101031	-	327	WP_004152639.1	hypothetical protein	-
EYB55_RS28810	101028..101756	-	729	WP_014386162.1	plasmid SOS inhibition protein A	-
EYB55_RS28815	101753..102184	-	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / aph(3')-Ia / tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15	-	1..225575	225575
	-	flank	IS/Tn	-	-	99127..100095	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T119606 WP_014386165.1 NZ_CP036337:98388-98804 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T119606 NZ_CP036337:98388-98804 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT119606 WP_032408893.1 NZ_CP036337:98161-98391 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT119606 NZ_CP036337:98161-98391 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA