Detailed information of TA system TA119605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 74266..75011
• Replicon: plasmid pIncFIBK
• Accession: NZ_CP036337
• Organism: Klebsiella pneumoniae strain BP327

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: EYB55_RS28645
• Protein ID: WP_032408901.1
• Coordinates: 74520..75011 (+)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: EYB55_RS28640
• Protein ID: WP_014386183.1
• Coordinates: 74266..74532 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYB55_RS28580	69821..70234	-	414	WP_013023817.1	helix-turn-helix domain-containing protein	-
EYB55_RS28585	70235..70513	-	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
EYB55_RS28590	70503..70823	-	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EYB55_RS28595	70904..71128	-	225	WP_014386189.1	hypothetical protein	-
EYB55_RS28600	71139..71351	-	213	WP_014386188.1	hypothetical protein	-
EYB55_RS28605	71411..71737	-	327	WP_014386187.1	hypothetical protein	-
EYB55_RS28620	72374..72724	-	351	WP_014386186.1	hypothetical protein	-
EYB55_RS28625	72721..72993	-	273	WP_032408902.1	hypothetical protein	-
EYB55_RS28630	73183..73668	+	486	WP_014386185.1	hypothetical protein	-
EYB55_RS28635	73911..74069	-	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
EYB55_RS28640	74266..74532	+	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
EYB55_RS28645	74520..75011	+	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
EYB55_RS28650	75452..75703	-	252	WP_032408900.1	aldo/keto reductase	-
EYB55_RS28655	75900..77492	-	1593	Protein_86	IS66 family transposase	-
EYB55_RS28660	77523..77873	-	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
EYB55_RS28665	77870..78310	-	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
EYB55_RS28670	78572..79327	-	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / aph(3')-Ia / tet(A) / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15	-	1..225575	225575

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T119605 WP_032408901.1 NZ_CP036337:74520-75011 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T119605 NZ_CP036337:74520-75011 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT119605 WP_014386183.1 NZ_CP036337:74266-74532 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT119605 NZ_CP036337:74266-74532 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6