Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 53090..53615 Replicon plasmid pIncFIA
Accession NZ_CP036329
Organism Klebsiella pneumoniae strain BA28434

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag C3E96_RS28360 Protein ID WP_001159868.1
Coordinates 53310..53615 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag C3E96_RS28355 Protein ID WP_000813634.1
Coordinates 53090..53308 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C3E96_RS28325 48483..48899 - 417 WP_001034046.1 type II toxin-antitoxin system VapC family toxin -
C3E96_RS28330 48896..49126 - 231 WP_001261278.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C3E96_RS28335 49391..49891 + 501 WP_000528931.1 hypothetical protein -
C3E96_RS28340 49904..50677 + 774 WP_000905949.1 hypothetical protein -
C3E96_RS28345 50844..51977 + 1134 WP_000545987.1 DUF3800 domain-containing protein -
C3E96_RS28350 52011..52523 - 513 WP_000151784.1 hypothetical protein -
C3E96_RS28355 53090..53308 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C3E96_RS28360 53310..53615 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
C3E96_RS28365 53616..54422 + 807 WP_000016982.1 site-specific integrase -
C3E96_RS28370 55197..55952 + 756 WP_000852146.1 replication initiation protein RepE -
C3E96_RS28375 56539..57705 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / mph(A) / sul1 / qacE / aadA2 / dfrA12 / sitABCD iucA / iucB / iucC / iucD / iutA 1..71246 71246


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T119585 WP_001159868.1 NZ_CP036329:53310-53615 [Klebsiella pneumoniae]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T119585 NZ_CP036329:53310-53615 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT119585 WP_000813634.1 NZ_CP036329:53090-53308 [Klebsiella pneumoniae]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT119585 NZ_CP036329:53090-53308 [Klebsiella pneumoniae]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References