Detailed information of TA system TA119549

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 3973518..3974197
• Replicon: chromosome
• Accession: NZ_CP036310
• Organism: Enterobacter hormaechei strain WCHEH090011

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: EKN58_RS19520
• Protein ID: WP_032608412.1
• Coordinates: 3973518..3973859 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: EKN58_RS19525
• Protein ID: WP_020456353.1
• Coordinates: 3973880..3974197 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKN58_RS19485	3969011..3969262	+	252	WP_032608418.1	hypothetical protein	-
EKN58_RS19490	3969335..3969559	+	225	WP_032608417.1	hypothetical protein	-
EKN58_RS19495	3969618..3969866	+	249	WP_032608415.1	bacteriocin immunity protein	-
EKN58_RS19500	3969981..3970352	+	372	WP_032608414.1	hypothetical protein	-
EKN58_RS19505	3970432..3970905	-	474	WP_072163380.1	SymE family type I addiction module toxin	-
EKN58_RS19510	3971206..3972075	+	870	WP_032608413.1	HNH endonuclease	-
EKN58_RS19515	3972256..3973263	-	1008	WP_020456355.1	restriction endonuclease	-
EKN58_RS19520	3973518..3973859	-	342	WP_032608412.1	TA system toxin CbtA family protein	Toxin
EKN58_RS19525	3973880..3974197	-	318	WP_020456353.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
EKN58_RS19530	3974215..3974436	-	222	WP_032608411.1	DUF987 domain-containing protein	-
EKN58_RS19535	3974445..3974924	-	480	WP_020456351.1	DNA repair protein RadC	-
EKN58_RS19540	3974967..3975185	-	219	WP_020456350.1	hypothetical protein	-
EKN58_RS19545	3975195..3975653	-	459	WP_032608410.1	antirestriction protein	-
EKN58_RS19550	3975759..3975959	-	201	WP_050596949.1	DUF905 family protein	-
EKN58_RS19555	3976087..3976953	-	867	WP_032608409.1	hypothetical protein	-
EKN58_RS19560	3977192..3977875	-	684	WP_020456346.1	DeoR family transcriptional regulator	-
EKN58_RS19565	3978084..3978908	-	825	Protein_3765	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3969011..4003619	34608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12747.73 Da
• Isoelectric Point: 9.0060

>T119549 WP_032608412.1 NZ_CP036310:c3973859-3973518 [Enterobacter hormaechei]
MKTLPATTPQAVTLCLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
NWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T119549 NZ_CP036310:c3973859-3973518 [Enterobacter hormaechei]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11846.39 Da
• Isoelectric Point: 5.5403

>AT119549 WP_020456353.1 NZ_CP036310:c3974197-3973880 [Enterobacter hormaechei]
MSNTTWGLQRDITPRLGARLVQEGNWLHYLADRASITGKFSEAECLKLDVVFPNFISQLESMLTTGEMNPSHAYCVTLYH
NGFTCEADTLGSYGYVYIAVYPTQR

Nucleotide

• Length: 318 bp

>AT119549 NZ_CP036310:c3974197-3973880 [Enterobacter hormaechei]
ATGAGCAACACCACATGGGGACTGCAGCGGGATATCACGCCGCGCCTGGGAGCACGTCTGGTTCAGGAGGGCAACTGGCT
GCACTATTTGGCTGACCGAGCCAGTATTACCGGTAAATTCAGCGAAGCAGAATGTCTTAAGCTGGATGTGGTATTTCCGA
ATTTTATCAGCCAGTTGGAGTCAATGCTTACCACTGGTGAAATGAACCCTAGCCATGCCTATTGTGTCACCCTGTACCAC
AATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGCCGTTTACCCCACTCAACGTTAA