Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7033..7676 Replicon plasmid pKPC2_020098
Accession NZ_CP036306
Organism Klebsiella pneumoniae strain WCHKP020098

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag C6M05_RS27445 Protein ID WP_001044770.1
Coordinates 7260..7676 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag C6M05_RS27440 Protein ID WP_001261282.1
Coordinates 7033..7263 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M05_RS27395 2313..2492 + 180 WP_006788217.1 hypothetical protein -
C6M05_RS27400 2996..3790 - 795 WP_004197635.1 site-specific integrase -
C6M05_RS27405 3988..5004 - 1017 WP_017899884.1 hypothetical protein -
C6M05_RS27410 5015..5329 - 315 WP_053389906.1 hypothetical protein -
C6M05_RS27415 5356..5751 - 396 WP_017899885.1 hypothetical protein -
C6M05_RS27420 5920..6225 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
C6M05_RS27425 6227..6445 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
C6M05_RS27435 6615..7076 - 462 WP_160866775.1 hypothetical protein -
C6M05_RS27440 7033..7263 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
C6M05_RS27445 7260..7676 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
C6M05_RS27450 7750..9312 + 1563 WP_004206609.1 AAA family ATPase -
C6M05_RS27455 9297..10319 + 1023 WP_000361404.1 helicase UvrD -
C6M05_RS27460 10575..11272 + 698 Protein_14 IS1 family transposase -
C6M05_RS27465 11301..11573 + 273 Protein_15 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 - 1..149519 149519


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T119525 WP_001044770.1 NZ_CP036306:7260-7676 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T119525 NZ_CP036306:7260-7676 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT119525 WP_001261282.1 NZ_CP036306:7033-7263 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT119525 NZ_CP036306:7033-7263 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References