Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 5920..6445 Replicon plasmid pKPC2_020098
Accession NZ_CP036306
Organism Klebsiella pneumoniae strain WCHKP020098

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag C6M05_RS27420 Protein ID WP_013023785.1
Coordinates 5920..6225 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag C6M05_RS27425 Protein ID WP_001568025.1
Coordinates 6227..6445 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C6M05_RS27390 1567..2193 + 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -
C6M05_RS27395 2313..2492 + 180 WP_006788217.1 hypothetical protein -
C6M05_RS27400 2996..3790 - 795 WP_004197635.1 site-specific integrase -
C6M05_RS27405 3988..5004 - 1017 WP_017899884.1 hypothetical protein -
C6M05_RS27410 5015..5329 - 315 WP_053389906.1 hypothetical protein -
C6M05_RS27415 5356..5751 - 396 WP_017899885.1 hypothetical protein -
C6M05_RS27420 5920..6225 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
C6M05_RS27425 6227..6445 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
C6M05_RS27435 6615..7076 - 462 WP_160866775.1 hypothetical protein -
C6M05_RS27440 7033..7263 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
C6M05_RS27445 7260..7676 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin -
C6M05_RS27450 7750..9312 + 1563 WP_004206609.1 AAA family ATPase -
C6M05_RS27455 9297..10319 + 1023 WP_000361404.1 helicase UvrD -
C6M05_RS27460 10575..11272 + 698 Protein_14 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 - 1..149519 149519


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T119524 WP_013023785.1 NZ_CP036306:c6225-5920 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T119524 NZ_CP036306:c6225-5920 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT119524 WP_001568025.1 NZ_CP036306:c6445-6227 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT119524 NZ_CP036306:c6445-6227 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References