Detailed information of TA system TA119505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 3006..3586
• Replicon: plasmid p2_015093
• Accession: NZ_CP036303
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP015093

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: CLQ99_RS29945
• Protein ID: WP_071177730.1
• Coordinates: 3272..3586 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: CLQ99_RS29940
• Protein ID: WP_000093040.1
• Coordinates: 3006..3284 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ99_RS29925	1579..1824	+	246	WP_032440458.1	hypothetical protein	-
CLQ99_RS29930	2098..2469	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
CLQ99_RS29935	2466..2672	+	207	Protein_3	TonB family protein	-
CLQ99_RS29940	3006..3284	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
CLQ99_RS29945	3272..3586	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CLQ99_RS29950	3750..4178	+	429	WP_001140599.1	hypothetical protein	-
CLQ99_RS29955	4204..4383	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
CLQ99_RS29960	4410..4940	-	531	WP_071177729.1	hypothetical protein	-
CLQ99_RS29965	4947..5678	-	732	WP_071177728.1	replication-relaxation family protein	-
CLQ99_RS29970	5678..7642	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T119505 WP_071177730.1 NZ_CP036303:3272-3586 [Klebsiella pneumoniae subsp. pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T119505 NZ_CP036303:3272-3586 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCCACAGGTAACGATTTCCGCGCTGGCACAGCGCGATTTACAACGCCTTCAGGACTTTCTCAAAACCAAAAATCGGCT
GGCAGCCAGAAAAGGAGGTGAGGTGATCGTCCGGGCTATCCAGCAACTGAAAACATTGCCAGACATTGGCCGCCCGGTGC
CGTTTCTGCCGCTGGAATATAAGGAACTGGTGATCGGGTTTGGCGACAGTGGCTATGTGATGCTCTACCGCCACGACCGG
GAAATGGACCAGATTGTGATCGTCACCGTCAGGCATCAGAAAGAATCCGGGTATCCGGGGGCAGACAGCCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT119505 WP_000093040.1 NZ_CP036303:3006-3284 [Klebsiella pneumoniae subsp. pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT119505 NZ_CP036303:3006-3284 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTCATCAGCAACATCCATCAGGCTCGATGAAGAACTTAAGGACCGGCTGAAAACGCTGGCCGATGACCGGCACCGCTC
AGCGCACGCGCTAATGCTGGAGGCAATCACCGAATACATCGACCGGGAAGAGAAGCGCAGCCAGTACCTGCGGGACGGAC
AGGCCGCATGGCAGCACTATCAGGAAACGGGTCTGCACCTTACTGCGGAAGAAGCGGAAGCCTGGATAAGTACCTGGGGA
ACTGAGAACGAGCAGGATGCTCCGCCATGCCACAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1