Detailed information of TA system TA119504

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 182883..183526
• Replicon: plasmid p1_015093
• Accession: NZ_CP036302
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain WCHKP015093

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: CLQ99_RS29755
• Protein ID: WP_014386165.1
• Coordinates: 183110..183526 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: CLQ99_RS29750
• Protein ID: WP_032408893.1
• Coordinates: 182883..183113 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CLQ99_RS29720	177956..178951	-	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-
CLQ99_RS29725	178958..180106	-	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
CLQ99_RS29730	180277..181434	-	1158	WP_032408895.1	acetylornithine deacetylase	-
CLQ99_RS29735	181450..182124	-	675	WP_014386166.1	DUF1028 domain-containing protein	-
CLQ99_RS29740	182129..182563	-	435	WP_000103648.1	RidA family protein	-
CLQ99_RS29750	182883..183113	+	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CLQ99_RS29755	183110..183526	+	417	WP_014386165.1	PIN domain-containing protein	Toxin
CLQ99_RS29760	183849..184817	+	969	WP_074422984.1	IS5 family transposase	-
CLQ99_RS29765	184997..185371	-	375	WP_032408891.1	hypothetical protein	-
CLQ99_RS29770	185427..185753	-	327	WP_013023807.1	hypothetical protein	-
CLQ99_RS29775	185750..186478	-	729	WP_001568058.1	plasmid SOS inhibition protein A	-
CLQ99_RS29780	186475..186906	-	432	WP_001568057.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(3')-Ia / dfrA12 / aadA2 / qacE / sul1 / mph(A)	-	1..205652	205652
	-	flank	IS/Tn	-	-	183849..184817	968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T119504 WP_014386165.1 NZ_CP036302:183110-183526 [Klebsiella pneumoniae subsp. pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T119504 NZ_CP036302:183110-183526 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT119504 WP_032408893.1 NZ_CP036302:182883-183113 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT119504 NZ_CP036302:182883-183113 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA