Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 140416..141059 Replicon plasmid pKPC2_015093
Accession NZ_CP036301
Organism Klebsiella pneumoniae subsp. pneumoniae strain WCHKP015093

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag CLQ99_RS28555 Protein ID WP_001044770.1
Coordinates 140643..141059 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag CLQ99_RS28550 Protein ID WP_001261282.1
Coordinates 140416..140646 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CLQ99_RS28515 135639..136418 - 780 WP_013214009.1 site-specific integrase -
CLQ99_RS28520 136602..137606 - 1005 WP_011977814.1 hypothetical protein -
CLQ99_RS28525 137636..137839 - 204 WP_011977813.1 hypothetical protein -
CLQ99_RS28530 137885..138406 - 522 WP_013214008.1 hypothetical protein -
CLQ99_RS28535 138464..138856 - 393 WP_011977811.1 hypothetical protein -
CLQ99_RS28540 138893..139837 - 945 WP_011977810.1 hypothetical protein -
CLQ99_RS28545 139998..140459 - 462 WP_014343465.1 hypothetical protein -
CLQ99_RS28550 140416..140646 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
CLQ99_RS28555 140643..141059 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
CLQ99_RS28560 141133..142695 + 1563 WP_004206609.1 AAA family ATPase -
CLQ99_RS28565 142680..143702 + 1023 WP_000361404.1 helicase UvrD -
CLQ99_RS28570 143958..144655 + 698 Protein_184 IS1 family transposase -
CLQ99_RS28575 144684..144956 + 273 Protein_185 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / blaCTX-M-65 / blaTEM-1B / rmtB - 1..154724 154724


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T119499 WP_001044770.1 NZ_CP036301:140643-141059 [Klebsiella pneumoniae subsp. pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T119499 NZ_CP036301:140643-141059 [Klebsiella pneumoniae subsp. pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT119499 WP_001261282.1 NZ_CP036301:140416-140646 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT119499 NZ_CP036301:140416-140646 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References