Detailed information of TA system TA119476

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 3695471..3696066
• Replicon: chromosome
• Accession: NZ_CP036294
• Organism: Alcaligenes faecalis strain AN70

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: EYC51_RS17085
• Protein ID: WP_131071734.1
• Coordinates: 3695761..3696066 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EYC51_RS17080
• Protein ID: WP_131071733.1
• Coordinates: 3695471..3695764 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYC51_RS17035	3690506..3691597	+	1092	WP_131071726.1	hypothetical protein	-
EYC51_RS17040	3691608..3691976	+	369	WP_131071727.1	hypothetical protein	-
EYC51_RS17045	3692130..3692615	+	486	WP_131071728.1	KilA-N domain-containing protein	-
EYC51_RS17050	3692653..3693054	+	402	WP_131071729.1	hypothetical protein	-
EYC51_RS17055	3693054..3693371	+	318	WP_131071730.1	hypothetical protein	-
EYC51_RS17060	3693407..3693778	+	372	WP_207307413.1	hypothetical protein	-
EYC51_RS17065	3693756..3694130	+	375	WP_131071731.1	hypothetical protein	-
EYC51_RS17070	3694140..3694883	+	744	WP_131071732.1	phage tail protein	-
EYC51_RS17075	3694880..3695464	+	585	WP_207307414.1	hypothetical protein	-
EYC51_RS17080	3695471..3695764	-	294	WP_131071733.1	putative addiction module antidote protein	Antitoxin
EYC51_RS17085	3695761..3696066	-	306	WP_131071734.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EYC51_RS17090	3696178..3696756	+	579	WP_131071735.1	hypothetical protein	-
EYC51_RS17095	3696815..3699400	+	2586	WP_131071736.1	tape measure protein	-
EYC51_RS17100	3699397..3699861	+	465	WP_131071737.1	hypothetical protein	-
EYC51_RS17105	3699858..3700340	+	483	WP_131071738.1	DUF1833 family protein	-
EYC51_RS17110	3700337..3700741	+	405	WP_131071739.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(A) / aph(3'')-Ib / aph(6)-Id / sul1 / qacE / catB3 / blaOXA-21 / aac(6')-IIa	-	3680853..3918986	238133

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (5-94)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11463.17 Da
• Isoelectric Point: 10.3556

>T119476 WP_131071734.1 NZ_CP036294:c3696066-3695761 [Alcaligenes faecalis]
MNTINRTETFSTWLAGLKDLKARAKIVVRIKQAGQGNFGDVKPISDGVWEMRVHFGPGYRLYYAREGRVVYLLLSGGDKS
TQKQDIKTAVAMWKQIQEDQS

Nucleotide

• Length: 306 bp

>T119476 NZ_CP036294:c3696066-3695761 [Alcaligenes faecalis]
ATGAACACGATCAACCGCACCGAAACATTCAGCACTTGGCTAGCAGGGTTGAAAGACCTCAAGGCCAGGGCGAAGATCGT
GGTTCGCATCAAACAAGCCGGTCAAGGGAATTTCGGCGACGTAAAGCCGATTAGCGATGGCGTCTGGGAGATGCGTGTCC
ACTTCGGCCCAGGCTATCGCCTCTACTATGCCCGTGAAGGCCGTGTGGTGTATCTCTTGCTCAGCGGCGGCGACAAATCC
ACCCAAAAGCAAGACATCAAGACCGCCGTCGCAATGTGGAAACAAATCCAGGAGGATCAGTCATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10322.92 Da
• Isoelectric Point: 4.8359

>AT119476 WP_131071733.1 NZ_CP036294:c3695764-3695471 [Alcaligenes faecalis]
MTTIKIAPFDAAEYLDSEEAIAEYLSAALEEDDPALFLSALADVVKARGMTKMAKDAGVGRESLYKALAPGAKPRYDTVL
KLARAAGVKLTVEPLHA

Nucleotide

• Length: 294 bp

>AT119476 NZ_CP036294:c3695764-3695471 [Alcaligenes faecalis]
ATGACCACCATCAAAATAGCCCCCTTCGACGCTGCCGAATACCTTGACAGCGAAGAGGCCATCGCCGAATACCTATCCGC
TGCGTTGGAAGAAGACGATCCCGCCCTCTTCCTGTCTGCACTTGCTGATGTAGTTAAAGCCAGAGGCATGACCAAAATGG
CGAAAGACGCTGGCGTTGGTCGGGAGAGCCTATACAAAGCCCTCGCCCCTGGTGCAAAGCCTCGATATGACACGGTGCTG
AAACTAGCTCGTGCTGCCGGGGTCAAGCTTACGGTGGAACCCCTTCACGCCTGA