Detailed information of TA system TA119473

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 5575418..5576076
• Replicon: chromosome
• Accession: NZ_CP036291
• Organism: Pirellulimonas nuda strain Pla175

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Pla175_RS21650
• Protein ID: WP_145290536.1
• Coordinates: 5575657..5576076 (+)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Pla175_RS21645
• Protein ID: WP_145290532.1
• Coordinates: 5575418..5575660 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Pla175_RS21625	5570990..5571379	+	390	WP_145290522.1	hypothetical protein	-
Pla175_RS21630	5571697..5572179	+	483	WP_145290525.1	hypothetical protein	-
Pla175_RS21635	5572377..5573795	-	1419	WP_197527055.1	type I glutamate--ammonia ligase	-
Pla175_RS21640	5574060..5575049	+	990	WP_145290528.1	serine/threonine protein kinase	-
Pla175_RS21645	5575418..5575660	+	243	WP_145290532.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
Pla175_RS21650	5575657..5576076	+	420	WP_145290536.1	type II toxin-antitoxin system VapC family toxin	Toxin
Pla175_RS21655	5576167..5576496	+	330	WP_145290541.1	hypothetical protein	-
Pla175_RS21660	5577043..5578587	+	1545	WP_197527056.1	sulfatase	-
Pla175_RS21665	5579081..5579524	+	444	WP_145290547.1	hypothetical protein	-
Pla175_RS21670	5579457..5580587	+	1131	WP_145284379.1	ISAs1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5579457..5580587	1130

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-64)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14949.16 Da
• Isoelectric Point: 7.2064

>T119473 WP_145290536.1 NZ_CP036291:5575657-5576076 [Pirellulimonas nuda]
VKVLLDTNVVSEARKPRGNPGVRRRLAELDEFNQFLSVVSIGEIAYGVARLARGKLRDALELWLAQAERSFADRLLPVDA
QVAGLWGELAARSAAAGGTISLADGLIAATAIHHGMRLMTRNVADFASTGGMLINPWEE

Nucleotide

• Length: 420 bp

>T119473 NZ_CP036291:5575657-5576076 [Pirellulimonas nuda]
GTGAAGGTGTTGCTCGATACGAATGTCGTCTCCGAAGCACGCAAGCCGCGCGGGAACCCCGGCGTACGCAGGCGGCTGGC
AGAGCTGGACGAATTCAATCAGTTCTTAAGCGTGGTCTCGATTGGCGAGATCGCTTACGGCGTGGCGCGGCTTGCCCGCG
GCAAACTCCGCGATGCCCTCGAGCTCTGGCTCGCCCAAGCAGAACGGAGTTTCGCAGACAGGCTCCTCCCGGTCGATGCC
CAGGTCGCCGGGCTCTGGGGCGAACTGGCAGCGCGATCCGCGGCGGCCGGTGGCACGATCTCGCTGGCCGATGGGCTGAT
CGCCGCCACCGCCATCCACCACGGCATGAGGTTGATGACCCGCAACGTGGCGGATTTTGCGTCGACGGGGGGGATGCTGA
TCAACCCGTGGGAGGAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9092.36 Da
• Isoelectric Point: 4.6283

>AT119473 WP_145290532.1 NZ_CP036291:5575418-5575660 [Pirellulimonas nuda]
MEWKLAEAKNKLSELVTRATSEGPQTIRRNGVGVIVVAEETYLELTGQRPSFKQWLLEGPRIDDLELPSRDPSPMREVDL

Nucleotide

• Length: 243 bp

>AT119473 NZ_CP036291:5575418-5575660 [Pirellulimonas nuda]
ATGGAATGGAAATTAGCCGAGGCCAAGAATAAGCTCAGCGAGCTTGTGACCCGGGCCACGTCCGAAGGGCCTCAAACGAT
TCGGCGAAACGGGGTGGGCGTAATCGTTGTGGCGGAAGAGACGTACTTGGAGCTGACCGGACAGCGTCCCAGTTTCAAGC
AGTGGCTGCTGGAAGGCCCCCGCATCGACGACCTAGAGTTGCCGAGCCGCGACCCATCGCCGATGCGAGAGGTCGACCTG
TGA