Detailed information of TA system TA119463

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 7533903..7534935
• Replicon: chromosome
• Accession: NZ_CP036274
• Organism: Anatilimnocola aggregata strain ETA_A8

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: ETAA8_RS28635
• Protein ID: WP_145096949.1
• Coordinates: 7534366..7534935 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: ETAA8_RS28630
• Protein ID: WP_145096947.1
• Coordinates: 7533903..7534364 (+)
• Length: 154 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ETAA8_RS35025	7529524..7529664	-	141	WP_202921330.1	hypothetical protein	-
ETAA8_RS28615	7529837..7531405	+	1569	WP_202921331.1	tetratricopeptide repeat protein	-
ETAA8_RS28620	7531570..7532445	+	876	WP_145096941.1	transglutaminase family protein	-
ETAA8_RS28625	7532426..7533286	+	861	WP_145096944.1	transglutaminase family protein	-
ETAA8_RS28630	7533903..7534364	+	462	WP_145096947.1	helix-turn-helix domain-containing protein	Antitoxin
ETAA8_RS28635	7534366..7534935	+	570	WP_145096949.1	PIN domain-containing protein	Toxin
ETAA8_RS28640	7535049..7535690	-	642	WP_145096952.1	uracil phosphoribosyltransferase	-
ETAA8_RS28645	7536198..7536971	+	774	WP_145096956.1	hypothetical protein	-
ETAA8_RS28650	7536992..7538038	+	1047	WP_145096959.1	glycosyltransferase family 9 protein	-
ETAA8_RS28655	7538415..7539245	+	831	WP_145096962.1	flagellar hook-basal body protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (83-128)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21730.83 Da
• Isoelectric Point: 5.3250

>T119463 WP_145096949.1 NZ_CP036274:7534366-7534935 [Anatilimnocola aggregata]
MSKFTVVYDACVLYPAPLRDFLMHLAMTDLFRAKWTNKIHEEWISNLLLNRDDLTRERLERTRDLMNQHVRDCLVEDFEH
FEKDLELPDPNDRHVLAAAIKARADAIITINLKDFPDSTLSKYGIEAMHPDDFINSQIGLSSAAVCGAAKRQRESLKNPP
KSVEEYLQTLEAQGLPQTIAALRYYAELI

Nucleotide

• Length: 570 bp

>T119463 NZ_CP036274:7534366-7534935 [Anatilimnocola aggregata]
TTGAGTAAGTTCACTGTCGTCTACGATGCCTGCGTCCTTTATCCGGCACCGTTGCGCGATTTCCTCATGCACTTGGCTAT
GACGGATTTATTTCGCGCGAAGTGGACGAACAAGATTCATGAAGAATGGATTTCGAATCTGCTCCTCAATCGTGATGACC
TGACAAGGGAAAGGCTGGAGCGAACACGAGACTTGATGAATCAGCACGTCCGTGATTGCCTGGTCGAAGATTTTGAGCAC
TTTGAGAAAGATCTTGAGCTTCCAGACCCCAACGACAGACACGTCCTCGCAGCGGCAATTAAAGCCCGAGCAGATGCAAT
TATTACGATAAACCTCAAGGACTTTCCCGATTCGACCTTGTCGAAATATGGCATCGAAGCAATGCATCCTGATGACTTCA
TTAATTCCCAAATCGGCTTGTCGAGTGCTGCCGTTTGTGGGGCAGCAAAACGGCAGCGAGAGAGCTTGAAGAACCCACCA
AAATCCGTTGAAGAGTATCTCCAAACGCTGGAAGCCCAAGGGCTTCCGCAAACGATTGCTGCTCTTCGATATTATGCAGA
GTTAATTTGA

Antitoxin

Protein

• Length: 154 a.a.
• Molecular weight: 16960.65 Da
• Isoelectric Point: 7.3607

>AT119463 WP_145096947.1 NZ_CP036274:7533903-7534364 [Anatilimnocola aggregata]
MSALTDKLETVTPTEHDATLAKKSGIQIARAMGKTARDLRVKIEGDDAETIELPHAALRLLANILAQMAQGNAFTVIPVH
AELTTQQAAEVLNVSRPFVIGLITNGKLPCRQVGTHRRILFRDIMNFKNKIDEDRMKVLDELAKEAQELNMGY

Nucleotide

• Length: 462 bp

>AT119463 NZ_CP036274:7533903-7534364 [Anatilimnocola aggregata]
ATGAGCGCATTAACCGACAAGCTGGAAACTGTCACACCAACTGAACATGATGCGACCCTAGCCAAGAAATCTGGCATACA
GATTGCGCGCGCCATGGGGAAGACCGCCCGTGATTTGCGTGTGAAAATCGAAGGGGACGATGCCGAGACAATCGAATTAC
CCCACGCTGCGCTCCGATTGCTGGCAAACATTCTTGCTCAAATGGCACAGGGAAACGCGTTTACAGTCATTCCTGTTCAT
GCAGAACTAACCACTCAGCAGGCAGCCGAGGTGCTAAACGTGTCGCGGCCTTTCGTAATCGGACTAATAACAAATGGAAA
ACTCCCCTGCCGCCAGGTTGGAACCCACCGGAGAATTTTGTTCCGCGACATCATGAATTTCAAGAACAAGATCGACGAAG
ACAGGATGAAAGTTCTGGATGAGTTGGCCAAAGAAGCGCAAGAGTTGAACATGGGTTACTAA