Detailed information of TA system TA119430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 45564..46183
• Replicon: plasmid pR712_02
• Accession: NZ_CP036248
• Organism: Enterococcus faecalis R712

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: EYB36_RS00690
• Protein ID: WP_000241511.1
• Coordinates: 45564..45926 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: EYB36_RS00695
• Protein ID: WP_000245205.1
• Coordinates: 45920..46183 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYB36_RS00675	40974..42905	-	1932	WP_002367772.1	sucrose-specific PTS transporter subunit IIBC	-
EYB36_RS00680	43096..44535	+	1440	WP_002390192.1	sucrose-6-phosphate hydrolase	-
EYB36_RS00685	44537..45499	+	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
EYB36_RS00690	45564..45926	-	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EYB36_RS00695	45920..46183	-	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
EYB36_RS00700	46681..47337	-	657	WP_000411487.1	hypothetical protein	-
EYB36_RS00705	48588..49229	-	642	WP_000406546.1	hypothetical protein	-
EYB36_RS00710	49611..50924	-	1314	WP_002383787.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / aph(3')-III / ant(6)-Ia	prgB/asc10	1..97950	97950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T119430 WP_000241511.1 NZ_CP036248:c45926-45564 [Enterococcus faecalis R712]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T119430 NZ_CP036248:c45926-45564 [Enterococcus faecalis R712]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT119430 WP_000245205.1 NZ_CP036248:c46183-45920 [Enterococcus faecalis R712]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT119430 NZ_CP036248:c46183-45920 [Enterococcus faecalis R712]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1