Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 20635..21206 Replicon plasmid pR712_02
Accession NZ_CP036248
Organism Enterococcus faecalis R712

Toxin (Protein)


Gene name mazF Uniprot ID R3HR30
Locus tag EYB36_RS00555 Protein ID WP_001278770.1
Coordinates 20865..21206 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A6B1XRC3
Locus tag EYB36_RS00550 Protein ID WP_000958277.1
Coordinates 20635..20865 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYB36_RS00525 15903..16505 - 603 WP_001062586.1 recombinase family protein -
EYB36_RS15650 16519..16689 - 171 WP_000713595.1 hypothetical protein -
EYB36_RS00530 17012..17692 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EYB36_RS00535 17820..18779 + 960 WP_000222577.1 IS30-like element IS1252 family transposase -
EYB36_RS00540 19212..19784 - 573 WP_000170424.1 recombinase family protein -
EYB36_RS00545 19800..20405 - 606 WP_000599739.1 cell filamentation protein -
EYB36_RS00550 20635..20865 + 231 WP_000958277.1 hypothetical protein Antitoxin
EYB36_RS00555 20865..21206 + 342 WP_001278770.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EYB36_RS00560 21318..22256 + 939 WP_001053446.1 hypothetical protein -
EYB36_RS00565 22503..23105 + 603 WP_000892301.1 Fic family protein -
EYB36_RS00570 23818..24084 + 267 WP_000246197.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
EYB36_RS00575 24077..24352 + 276 WP_002402695.1 type II toxin-antitoxin system YafQ family toxin -
EYB36_RS00580 25231..25557 - 327 WP_000606299.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid erm(B) / aph(3')-III / ant(6)-Ia prgB/asc10 1..97950 97950


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13220.44 Da        Isoelectric Point: 8.8595

>T119429 WP_001278770.1 NZ_CP036248:20865-21206 [Enterococcus faecalis R712]
MSDEKKYIPKKGDIVWIDFDPSAGKEIQKRRPGLVVSRYEFNRKTMFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Download         Length: 342 bp

>T119429 NZ_CP036248:20865-21206 [Enterococcus faecalis R712]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGGAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACAATGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCCACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGATTTTACAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8536.45 Da        Isoelectric Point: 4.2412

>AT119429 WP_000958277.1 NZ_CP036248:20635-20865 [Enterococcus faecalis R712]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWYDIEPGGRELF

Download         Length: 231 bp

>AT119429 NZ_CP036248:20635-20865 [Enterococcus faecalis R712]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAACAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGATCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGTATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R3HR30

Antitoxin

Source ID Structure
AlphaFold DB A0A6B1XRC3

References