Detailed information of TA system TA119428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 7231..8339
• Replicon: plasmid pR712_02
• Accession: NZ_CP036248
• Organism: Enterococcus faecalis R712

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: R3JIN1
• Locus tag: EYB36_RS00485
• Protein ID: WP_000233000.1
• Coordinates: 7231..8100 (-)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: EYB36_RS00490
• Protein ID: WP_000205227.1
• Coordinates: 8115..8339 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYB36_RS00450	2725..3462	-	738	WP_001038790.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
EYB36_RS00455	3587..3670	-	84	WP_001814874.1	23S rRNA methyltransferase attenuation leader peptide	-
EYB36_RS00460	3718..3801	-	84	Protein_4	peptide-binding protein	-
EYB36_RS00465	4211..5005	-	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
EYB36_RS00470	5098..5578	-	481	Protein_6	GNAT family N-acetyltransferase	-
EYB36_RS00475	5575..6483	-	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
EYB36_RS00480	6516..7250	-	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
EYB36_RS00485	7231..8100	-	870	WP_000233000.1	nucleotidyltransferase domain-containing protein	Toxin
EYB36_RS00490	8115..8339	-	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
EYB36_RS00495	8482..10044	-	1563	WP_000136908.1	recombinase family protein	-
EYB36_RS00500	10046..10462	-	417	WP_000323438.1	recombinase	-
EYB36_RS00505	10463..10759	-	297	WP_002360685.1	hypothetical protein	-
EYB36_RS00510	11152..11757	-	606	WP_000238804.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	erm(B) / aph(3')-III / ant(6)-Ia	prgB/asc10	1..97950	97950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32926.57 Da
• Isoelectric Point: 4.8781

>T119428 WP_000233000.1 NZ_CP036248:c8100-7231 [Enterococcus faecalis R712]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T119428 NZ_CP036248:c8100-7231 [Enterococcus faecalis R712]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGCATTAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAATATAAACCTGAAAAATATGCCGAA
AGAGTTAATCATATTTTTGAAGTACTCGGTCTTTCTCTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT119428 WP_000205227.1 NZ_CP036248:c8339-8115 [Enterococcus faecalis R712]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT119428 NZ_CP036248:c8339-8115 [Enterococcus faecalis R712]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: R3JIN1