Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 32837..33356 Replicon plasmid pR712_01
Accession NZ_CP036247
Organism Enterococcus faecalis R712

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag EYB36_RS00185 Protein ID WP_000588503.1
Coordinates 32837..33094 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag EYB36_RS00190 Protein ID WP_000388479.1
Coordinates 33087..33356 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYB36_RS00165 29601..31040 + 1440 WP_002367771.1 sucrose-6-phosphate hydrolase -
EYB36_RS00170 31042..32004 + 963 WP_002367770.1 LacI family DNA-binding transcriptional regulator -
EYB36_RS00175 32069..32431 - 363 WP_000241511.1 type II toxin-antitoxin system PemK/MazF family toxin -
EYB36_RS00180 32425..32688 - 264 WP_000245205.1 PbsX family transcriptional regulator -
EYB36_RS00185 32837..33094 - 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
EYB36_RS00190 33087..33356 - 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EYB36_RS00195 33487..33666 - 180 WP_001120991.1 hypothetical protein -
EYB36_RS00200 34047..34742 - 696 WP_002326819.1 hypothetical protein -
EYB36_RS00205 34804..35484 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -
EYB36_RS00210 35539..35736 + 198 Protein_43 recombinase family protein -
EYB36_RS00215 36015..36302 - 288 WP_000312841.1 type II toxin-antitoxin system RelE/ParE family toxin -
EYB36_RS00220 36292..36621 - 330 WP_000629052.1 hypothetical protein -
EYB36_RS00225 36926..37255 + 330 WP_000754864.1 metalloregulator ArsR/SmtB family transcription factor -
EYB36_RS00230 37542..38027 - 486 WP_000516404.1 glycopeptide resistance protein VanZ-A -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) / aac(6')-aph(2'') - 1..75109 75109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T119427 WP_000588503.1 NZ_CP036247:c33094-32837 [Enterococcus faecalis R712]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T119427 NZ_CP036247:c33094-32837 [Enterococcus faecalis R712]
ATGATTAAGGCTTGGTCTGATGATGCTTGGGATGATTATCTTTATTGGCATGAGCAAGGAAACAAAAGCAATATAAAAAA
GATTAACAAGTTAATAAAAGATATCGATCGTTCCCCCTTTGCTGGATTAGGAAAACCTGAGCCATTAAAGCATGATTTAT
CTGGAAAATGGTCCAGAAGAATTACAGATGAACATAGACTGATATATAGAGTTGAAAATGAAACGATATTTATTTATTCT
GCAAAAGATCACTATTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT119427 WP_000388479.1 NZ_CP036247:c33356-33087 [Enterococcus faecalis R712]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT119427 NZ_CP036247:c33356-33087 [Enterococcus faecalis R712]
ATGGAAGCAGTAGCTTATTCAAATTTCCGCCAAAATTTACGTAGTTATATGAAACAAGTTAATGAGGATGCTGAAACACT
TATTGTAACAAGTAAAGATGTAGAAGATACAGTTGTTGTATTATCAAAAAGAGATTATGATTCTATGCAAGAAACGTTGA
GAACACTTTCTAATAATTACGTCATGGAAAAAATTCGTCGAGGAGATGAACAATTCTCCAAAGGTGCATTTAAAACACAT
GACTTAATCGAGGTTGAATCTGATGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References