Detailed information of TA system TA119425

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 32069..32688
• Replicon: plasmid pR712_01
• Accession: NZ_CP036247
• Organism: Enterococcus faecalis R712

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: R3H4V2
• Locus tag: EYB36_RS00175
• Protein ID: WP_000241511.1
• Coordinates: 32069..32431 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3H5D1
• Locus tag: EYB36_RS00180
• Protein ID: WP_000245205.1
• Coordinates: 32425..32688 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EYB36_RS00160	27479..29410	-	1932	WP_002367772.1	sucrose-specific PTS transporter subunit IIBC	-
EYB36_RS00165	29601..31040	+	1440	WP_002367771.1	sucrose-6-phosphate hydrolase	-
EYB36_RS00170	31042..32004	+	963	WP_002367770.1	LacI family DNA-binding transcriptional regulator	-
EYB36_RS00175	32069..32431	-	363	WP_000241511.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EYB36_RS00180	32425..32688	-	264	WP_000245205.1	PbsX family transcriptional regulator	Antitoxin
EYB36_RS00185	32837..33094	-	258	WP_000588503.1	Txe/YoeB family addiction module toxin	-
EYB36_RS00190	33087..33356	-	270	WP_000388479.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EYB36_RS00195	33487..33666	-	180	WP_001120991.1	hypothetical protein	-
EYB36_RS00200	34047..34742	-	696	WP_002326819.1	hypothetical protein	-
EYB36_RS00205	34804..35484	+	681	WP_001015311.1	IS6-like element IS1216 family transposase	-
EYB36_RS00210	35539..35736	+	198	Protein_43	recombinase family protein	-
EYB36_RS00215	36015..36302	-	288	WP_000312841.1	type II toxin-antitoxin system RelE/ParE family toxin	-
EYB36_RS00220	36292..36621	-	330	WP_000629052.1	hypothetical protein	-
EYB36_RS00225	36926..37255	+	330	WP_000754864.1	metalloregulator ArsR/SmtB family transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	VanHAX / erm(B) / aac(6')-aph(2'')	-	1..75109	75109

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13585.78 Da
• Isoelectric Point: 9.6107

>T119425 WP_000241511.1 NZ_CP036247:c32431-32069 [Enterococcus faecalis R712]
MVKVPHQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVIVAPISSTKRNYPLYVSINPSYGMKTSGKVLLDQLT
TIDYEARQCVFLETAHEKLIDELLLKVRTVFQKVNKTNKF

Nucleotide

• Length: 363 bp

>T119425 NZ_CP036247:c32431-32069 [Enterococcus faecalis R712]
ATGGTAAAAGTTCCTCATCAAGGTGATATTTTATTGTTGAATACGGCTCCTAGATCAGGACATGAACAAACAGGTAAGAG
ACCGTATATTGTTTTAAGTCATGATATTATTGCAGATTACAGCAATGTTGTTATTGTAGCTCCTATATCAAGTACAAAAA
GAAACTATCCTTTATATGTTTCTATTAATCCTTCTTACGGAATGAAAACATCAGGTAAAGTATTATTAGATCAATTAACC
ACCATCGATTATGAGGCTAGACAATGCGTGTTTTTAGAAACAGCACACGAAAAATTAATCGATGAATTATTGCTAAAAGT
TAGAACCGTATTTCAAAAAGTAAATAAAACAAATAAATTTTAA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10059.46 Da
• Isoelectric Point: 5.1585

>AT119425 WP_000245205.1 NZ_CP036247:c32688-32425 [Enterococcus faecalis R712]
MVMLSIKKWGNSNGLRLPKSVMEYLQIHTEDKVKITQEESNGKKRLIIEAVDSDNDLTIEQLFENYKEEKVHVTIQDLGN
AVGNEKW

Nucleotide

• Length: 264 bp

>AT119425 NZ_CP036247:c32688-32425 [Enterococcus faecalis R712]
ATGGTAATGTTATCAATAAAAAAATGGGGCAATAGTAACGGTCTTAGACTTCCTAAATCTGTGATGGAATATCTCCAAAT
TCATACAGAAGATAAAGTGAAAATAACACAGGAGGAATCGAATGGTAAAAAACGATTAATTATTGAAGCTGTGGATTCTG
ATAATGATTTAACAATTGAACAATTGTTTGAAAATTATAAAGAAGAGAAGGTGCATGTAACTATTCAAGATTTAGGAAAT
GCGGTAGGAAATGAAAAATGGTAA

Structures

Toxin

• AlphaFold DB: R3H4V2

Antitoxin

• AlphaFold DB: R3H5D1