Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relE-dinJ/YafQ-DinJ
Location 1520..2054 Replicon plasmid pR712_01
Accession NZ_CP036247
Organism Enterococcus faecalis R712

Toxin (Protein)


Gene name relE Uniprot ID R3JX52
Locus tag EYB36_RS00030 Protein ID WP_002360769.1
Coordinates 1779..2054 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID R3H6P0
Locus tag EYB36_RS00025 Protein ID WP_002369771.1
Coordinates 1520..1786 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYB36_RS00010 145..563 + 419 Protein_1 single-stranded DNA-binding protein -
EYB36_RS00015 587..1172 + 586 Protein_2 thermonuclease family protein -
EYB36_RS00020 1169..1417 + 249 WP_002369773.1 hypothetical protein -
EYB36_RS00025 1520..1786 + 267 WP_002369771.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EYB36_RS00030 1779..2054 + 276 WP_002360769.1 type II toxin-antitoxin system YafQ family toxin Toxin
EYB36_RS00035 2858..3043 + 186 WP_002365983.1 hypothetical protein -
EYB36_RS00040 3028..3219 + 192 WP_002390216.1 DpnD/PcfM family protein -
EYB36_RS00045 3243..3833 + 591 WP_002360842.1 hypothetical protein -
EYB36_RS00050 3938..4717 + 780 WP_002390215.1 hypothetical protein -
EYB36_RS00055 4717..6060 + 1344 WP_002417363.1 PcfJ domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid VanHAX / erm(B) / aac(6')-aph(2'') - 1..75109 75109


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(7-87)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10732.58 Da        Isoelectric Point: 9.6918

>T119424 WP_002360769.1 NZ_CP036247:1779-2054 [Enterococcus faecalis R712]
MLEIFYTNQFKKDFKKAKKQGKNLEKLKEVLVLLQEQQTLPPKYKDHALTGNYIGTRECHIEPDWLLIYKIDGDKLILTL
ARIGSHSELFR

Download         Length: 276 bp

>T119424 NZ_CP036247:1779-2054 [Enterococcus faecalis R712]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAGTT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCACGCACTAACAGGAAATTATA
TAGGTACAAGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCTCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9804.15 Da        Isoelectric Point: 4.4791

>AT119424 WP_002369771.1 NZ_CP036247:1520-1786 [Enterococcus faecalis R712]
MATTKKKPIHVNVDENLKEEAEQLFDDLGLNMTSAITIFLKQSINEQAIPFMINKGNKETLQALKDIKEGNVHGGFSSVE
DLMEDLNA

Download         Length: 267 bp

>AT119424 NZ_CP036247:1520-1786 [Enterococcus faecalis R712]
TTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACTATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAACGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M2AEA7

Antitoxin

Source ID Structure
AlphaFold DB A0A0M2AED7

References