Detailed information of TA system TA119421

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 5083986..5084806
• Replicon: chromosome
• Accession: NZ_CP036245
• Organism: Escherichia coli strain S65EC

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: S65EC_RS26290
• Protein ID: WP_001054379.1
• Coordinates: 5084549..5084806 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: S65EC_RS26285
• Protein ID: WP_000123957.1
• Coordinates: 5083986..5084537 (-)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S65EC_RS26260	5079680..5080993	-	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-
S65EC_RS26265	5081005..5081283	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
S65EC_RS26270	5081280..5082401	-	1122	WP_000010833.1	M42 family metallopeptidase	-
S65EC_RS27085	5082646..5082762	-	117	Protein_4833	VOC family protein	-
S65EC_RS26275	5082800..5083060	-	261	WP_000077645.1	hypothetical protein	-
S65EC_RS26280	5083188..5083934	-	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
S65EC_RS26285	5083986..5084537	-	552	WP_000123957.1	N-acetyltransferase	Antitoxin
S65EC_RS26290	5084549..5084806	-	258	WP_001054379.1	YjhX family toxin	Toxin
S65EC_RS26295	5085183..5085347	-	165	Protein_4838	GNAT family N-acetyltransferase	-
S65EC_RS26300	5085380..5086525	-	1146	Protein_4839	IS3-like element ISEc52 family transposase	-
S65EC_RS26305	5086588..5087694	+	1107	Protein_4840	helicase YjhR	-
S65EC_RS26310	5088277..5089257	-	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T119421 WP_001054379.1 NZ_CP036245:c5084806-5084549 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T119421 NZ_CP036245:c5084806-5084549 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT119421 WP_000123957.1 NZ_CP036245:c5084537-5083986 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT119421 NZ_CP036245:c5084537-5083986 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030