Detailed information of TA system TA119417

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4730190..4730687
• Replicon: chromosome
• Accession: NZ_CP036245
• Organism: Escherichia coli strain S65EC

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: S65EC_RS24400
• Protein ID: WP_001173343.1
• Coordinates: 4730514..4730687 (+)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: S1NQ59
• Locus tag: S65EC_RS24395
• Protein ID: WP_001312331.1
• Coordinates: 4730190..4730486 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
S65EC_RS24385	4725547..4729476	+	3930	WP_001080129.1	autotransporter outer membrane beta-barrel domain-containing protein	-
S65EC_RS24390	4729690..4729962	+	273	WP_000405647.1	N-acetyltransferase	-
S65EC_RS24395	4730190..4730486	+	297	WP_001312331.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
S65EC_RS24400	4730514..4730687	+	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
S65EC_RS24405	4730806..4732323	-	1518	WP_000003806.1	lysine--tRNA ligase	-
S65EC_RS24410	4732560..4734017	-	1458	WP_000856826.1	dipeptide/tripeptide permease DtpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4714599..4761391	46792

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T119417 WP_001173343.1 NZ_CP036245:4730514-4730687 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T119417 NZ_CP036245:4730514-4730687 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11440.95 Da
• Isoelectric Point: 4.1705

>AT119417 WP_001312331.1 NZ_CP036245:4730190-4730486 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLSIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT119417 NZ_CP036245:4730190-4730486 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A7U9QI41

Antitoxin

• AlphaFold DB: S1NQ59