Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3211612..3212266 Replicon chromosome
Accession NZ_CP036245
Organism Escherichia coli strain S65EC

Toxin (Protein)


Gene name cptA Uniprot ID F4T2L4
Locus tag S65EC_RS16940 Protein ID WP_000244765.1
Coordinates 3211612..3212019 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID F4T2L5
Locus tag S65EC_RS16945 Protein ID WP_000354050.1
Coordinates 3212000..3212266 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S65EC_RS16920 3207569..3209302 - 1734 WP_000813195.1 single-stranded-DNA-specific exonuclease RecJ -
S65EC_RS16925 3209308..3210018 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
S65EC_RS16930 3210043..3210939 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
S65EC_RS16935 3211051..3211572 + 522 WP_001055874.1 flavodoxin FldB -
S65EC_RS16940 3211612..3212019 - 408 WP_000244765.1 protein YgfX Toxin
S65EC_RS16945 3212000..3212266 - 267 WP_000354050.1 FAD assembly factor SdhE Antitoxin
S65EC_RS16950 3212509..3213489 + 981 WP_000886084.1 tRNA-modifying protein YgfZ -
S65EC_RS16955 3213566..3214225 - 660 WP_000250275.1 hemolysin III family protein -
S65EC_RS16960 3214389..3214700 - 312 WP_001182956.1 N(4)-acetylcytidine aminohydrolase -
S65EC_RS16965 3214745..3216178 + 1434 WP_001296350.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16043.95 Da        Isoelectric Point: 11.5202

>T119411 WP_000244765.1 NZ_CP036245:c3212019-3211612 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLIPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T119411 NZ_CP036245:c3212019-3211612 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATTCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10535.07 Da        Isoelectric Point: 5.1599

>AT119411 WP_000354050.1 NZ_CP036245:c3212266-3212000 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRTFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT119411 NZ_CP036245:c3212266-3212000 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCACCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A454A7D7

Antitoxin

Source ID Structure
AlphaFold DB A0A061L3F4

References