Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2278150..2278981 Replicon chromosome
Accession NZ_CP036245
Organism Escherichia coli strain S65EC

Toxin (Protein)


Gene name yeeV Uniprot ID A0A066T988
Locus tag S65EC_RS12690 Protein ID WP_000854815.1
Coordinates 2278607..2278981 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A061Y7A8
Locus tag S65EC_RS12685 Protein ID WP_001280918.1
Coordinates 2278150..2278518 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S65EC_RS12630 2273239..2273985 + 747 WP_001016257.1 IS21-like element ISEc12 family helper ATPase IstB -
S65EC_RS12640 2274068..2274418 + 351 Protein_2248 hypothetical protein -
S65EC_RS12645 2274434..2274844 + 411 WP_000846703.1 hypothetical protein -
S65EC_RS12655 2275065..2275883 + 819 WP_001542275.1 DUF945 domain-containing protein -
S65EC_RS12660 2275883..2276128 + 246 WP_001164966.1 hypothetical protein -
S65EC_RS12665 2276222..2276695 + 474 WP_001542276.1 antirestriction protein -
S65EC_RS12670 2276711..2277187 + 477 WP_001186200.1 RadC family protein -
S65EC_RS12675 2277250..2277471 + 222 WP_000692345.1 DUF987 domain-containing protein -
S65EC_RS12680 2277490..2278134 + 645 WP_000086752.1 hypothetical protein -
S65EC_RS12685 2278150..2278518 + 369 WP_001280918.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
S65EC_RS12690 2278607..2278981 + 375 WP_000854815.1 type IV toxin-antitoxin system toxin CbtA Toxin
S65EC_RS12695 2278978..2279172 + 195 WP_000988600.1 hypothetical protein -
S65EC_RS12700 2279185..2279298 + 114 WP_001161660.1 DUF957 domain-containing protein -
S65EC_RS12705 2279587..2279733 - 147 Protein_2260 transposase domain-containing protein -
S65EC_RS12710 2279787..2279969 + 183 WP_001296208.1 hypothetical protein -
S65EC_RS12715 2280070..2280399 - 330 WP_000450409.1 DUF496 family protein -
S65EC_RS12720 2280571..2281629 - 1059 WP_001200889.1 FUSC family protein -
S65EC_RS12725 2281827..2282300 - 474 WP_001105368.1 DNA gyrase inhibitor SbmC -
S65EC_RS12730 2282419..2283585 - 1167 WP_001296209.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13829.83 Da        Isoelectric Point: 7.1326

>T119409 WP_000854815.1 NZ_CP036245:2278607-2278981 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTSDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T119409 NZ_CP036245:2278607-2278981 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCAGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13811.70 Da        Isoelectric Point: 6.4767

>AT119409 WP_001280918.1 NZ_CP036245:2278150-2278518 [Escherichia coli]
VSDKLHETNYPDDHNDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT119409 NZ_CP036245:2278150-2278518 [Escherichia coli]
GTGTCAGACAAACTCCATGAGACAAATTATCCCGACGACCATAACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A066T988

Antitoxin

Source ID Structure
AlphaFold DB A0A061Y7A8

References