Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 506744..507362 Replicon chromosome
Accession NZ_CP036245
Organism Escherichia coli strain S65EC

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag S65EC_RS03365 Protein ID WP_001291435.1
Coordinates 506744..506962 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag S65EC_RS03370 Protein ID WP_000344800.1
Coordinates 506988..507362 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
S65EC_RS03330 502031..502603 + 573 WP_000779831.1 YbaY family lipoprotein -
S65EC_RS03335 502634..502945 - 312 WP_000409908.1 MGMT family protein -
S65EC_RS03345 503324..503677 + 354 WP_000878135.1 DUF1428 family protein -
S65EC_RS03350 503719..505269 - 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
S65EC_RS03355 505433..505903 - 471 WP_000136192.1 YlaC family protein -
S65EC_RS03360 506019..506570 - 552 WP_000102539.1 maltose O-acetyltransferase -
S65EC_RS03365 506744..506962 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
S65EC_RS03370 506988..507362 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
S65EC_RS03375 507908..511057 - 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
S65EC_RS03380 511080..512273 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T119394 WP_001291435.1 NZ_CP036245:c506962-506744 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T119394 NZ_CP036245:c506962-506744 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT119394 WP_000344800.1 NZ_CP036245:c507362-506988 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT119394 NZ_CP036245:c507362-506988 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References